Biotinylated Recombinant Human PD-1 (CD279)-Fc Chimera (carrier-free)

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Other Names
Programmed Cell Death Protein 1, PD1, PDCD1, CD2791, PD1, PDCD1, CD279PD-1, PD 1, Programmed Cell Death Protein
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Human_PD-1_Fc_CF_Biotin_RECOM_1_092320
When human B7-H1 (PD-L1, CD274)-Fc chimera is immobilized at 1 μg/mL, biotinylated recombinant human PD-1 (CD279)-Fc chimera binds in a dose-dependent manner. The EC50 range for this effect is 25 - 100 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_PD-1_Fc_CF_Biotin_RECOM_1_092320
    When human B7-H1 (PD-L1, CD274)-Fc chimera is immobilized at 1 μg/mL, biotinylated recombinant human PD-1 (CD279)-Fc chimera binds in a dose-dependent manner. The EC50 range for this effect is 25 - 100 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_PD-1_Fc_CF_Biotin_RECOM_2_092320
    Stability Testing for Recombinant Human PD-1 (CD279)-Fc Chimera. Recombinant human PD-1 (CD279)-Fc chimera was aliquoted in PBS at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested in a binding assay with human B7-H1 (PD-L1, CD274)-Fc Chimera.
Cat # Size Price Quantity Avail. Save
789402 10 µg 76€
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789404 25 µg 156€
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789406 100 µg 432€
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Description

Programmed death-1 (PD-1) is a type I transmembrane protein initially isolated from apoptosis-induced cells by subtractive hybridization. The amino acid sequence of mouse PD-1 shares 59.4% identity to the human counterpart, and a putative tyrosine kinase-association motif is well conserved. Its extracellular region consists of a single Ig-like variable (IgV) domain. PD-1 is an inhibitory molecule expressed by activated B and T cells and has been implicated in immune tolerance. PD-L1 and PD-L2 are the ligands for PD-1 and their binding leads to the inhibition of T cell receptor-mediated lymphocyte proliferation and cytokine secretion. The PD-1/PD-L1 interaction suppresses immune responses against autoantigens and tumors, and plays an important role in the maintenance of peripheral immune tolerance. Interaction between PD-1 and PD-L1 promote tolerance by blocking TCR-mediated signaling. PD-L1 expression by tumor cells allow tumor progression by suppressing antitumor T-cell responses. Antibodies against PD-1 or PD-L1 lead to increased antitumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human PD-1, amino acid Leu25-Gln167 (Accession # Q15116.3), with a C-terminal hIgG1 Fc tag, was expressed in 293E cells.
Molecular Mass
The unlabeled 382 amino acid recombinant protein has a predicted molecular mass of approximately 42.8 kD. The DTT-reduced protein migrates at approximately 60 - 70 kD and and non-reduced protein migrates at approximately 100 - 120 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 µm filtered protein solution is in PBS, 5% glycerol.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When human B7-H1 (PD-L1, CD274)-Fc chimera is immobilized at 1 µg/mL, biotinylated recombinant human PD-1 (CD279)-Fc chimera binds in a dose-dependent manner. The EC50 range for this effect is 25 - 100 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Covalently linked homodimer, biotinylated via amines
Distribution

Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes

Function
Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self
Interaction
APC, monocytes, dendritic cells, stromal cell, cancer cells
Ligand/Receptor
B7-H1 (PD-L1, CD274) and B7-DC (PD-L2, CD273)
Bioactivity
Measured by its ability to bind recombinant human B7-H1 (PD-L1, CD274)-Fc Chimera
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Monocytes, T cells
Biology Area
Adaptive Immunity, Immuno-Oncology, Immunology
Molecular Family
Immune Checkpoint Receptors, Soluble Receptors
Antigen References

1. Ishida Y, et al. 1992. EMBO J. 11:3887-95.
2. Fife BT, et al. 2009. Nat Immunol. 10:1185-92.
3. Freeman GJ, et al. 2000. J Exp Med. 192:1027-34.
4. Latchman Y, et al. 2001. Nat Immunol. 2:261-8.
5. Iwai Y, et al. 2002. Proc Natl Acad Sci U S A. 99:12293-7.
6. Dai S, et al. 2014. Cell Immunol. 290:72-9.
7. Tan S, et al. 2017. Nature Commun. 8:14369.

Gene ID
5133 View all products for this Gene ID
UniProt
View information about PD-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.09.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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