- TUJ1 (See other available formats)
- Regulatory Status
- Other Names
- CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, tubulin beta-4 chain, class III beta-tubulin
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- This antibody was raised against microtubules derived from rat brain.
- Phosphate-buffered solution, pH 7.2, containing no preservatives solution.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICC - Quality tested
3D IHC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml is recommended. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Application Notes
Additional reported applications (for the relevant formats) include: flow cytometry4, immunofluorescence microscopy1-5,7, immunohistochemistry5,7, Western blotting8, and spatial biology (IBEX)9,10.
This antibody is well characterized and highly reactive to neuron specific Class III ß-tubulin (ßIII). TUJ1 does not identify ß-tubulin found in glial cells. TUJ1 recognizes an epitope located within the last 15 C-terminal residues8.
(PubMed link indicates BioLegend citation)
- Nishimura K, et al. 2017. PLoS One. 12(1): e0170568. (ICC)
- Jongbloets J, et al. 2017. Nat Commun. 8: 14666. (ICC) PubMed
- Liu W.J, et al. 2015. Eur J Histochem. 59(1): 2464. (ICC) PubMed
- Chintalapudi SR, et al. 2016. Front Aging Neursci. 8:93. (FC, ICC) PubMed
- Ambasudhan R, et al. 2011. Cell Stem Cell. 9(2):113. (IHC, ICC)
- Hu X., et al. 2006. Nature Neuroscicene. 9(12):1520. (WB) PubMed
- Zechner D., et al. 2003. Develop Biology. 258(2):406. (ICC, IHC)
- Lee MK, et al. 1990. Proc. Natl. Acad. Sci. USA 18:7195. (WB)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_2650635 (BioLegend Cat. No. 801207)
AB_2650636 (BioLegend Cat. No. 801208)
Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.
- Tubulin β 3 is a 450 amino acid protein with a molecular mass of ~50 kD.
Tissue distribution: central and peripheral nervous system.
Cellular distribution: cytosol, cytoskeleton and nucleus.
- Tubulin β 3 is the major constituent of microtubules, and plays a critical role in proper axon guidance and maintenance.
- Alpha tubulin, kinesin and dynein.
- Cell Type
- Mature Neurons, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Antigen References
1. Zhao X, et al. 2017. Med Sci Monit. 22: 3915.
2. Lebok P, et al. 2016. Oncol Lett. 11(3):1987.
3. Du J, et al. 2015. BMC Cancer. 15:536. PubMed
4. Rogue DM., et al. 2013. Clin Exp Metastasis. 31(1): 101.
5. Ploussard G, et al. 2010. Cancer Res. 70(22):9253. PubMed
- Gene ID
- 10381 View all products for this Gene ID
- View information about Tubulin beta-3 on UniProt.org
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.