Biotin anti-mouse IL-10 + Brilliant Violet 421™ Streptavidin

Immortalized mouse bone marrow-derived macrophages, treated with LPS (100 ng/mL for 24 h), were stained with Alexa Fluor® 594 Wheat Germ Agglutinin (red) and biotin anti-mouse IL-10 (clone JES5-16E3), followed by Brilliant Violet 421™ Streptavidin (light blue). Data provided by James Harris, Trinity College, Dublin.

Biotin anti-mouse CD272 (BTLA) + PE Streptavidin

C57BL/6 splenocytes stained with biotin anti-mouse CD272 (clone 8F4)(left) or biotin anti-mouse IgG1 isotype control (right), followed by PE Streptavidin and APC anti-mouse CD3e (clone 145-2C11).

Biotin anti-mouse CD56 + Brilliant Violet 421™ Streptavidin

Human NK cell line NK92 fixed and stained with biotin anti-CD56 (clone HCD56) and Brilliant Violet 421™Streptavidin (blue), phalloidin Alexa Fluor® 568 (red), and anti-perforin FITC (green). The first image is the DIC view (far left). The final image (far right) shows the merged fluorescence of the three stains. Data provided by Emily Mace and Jordan Orange, Children's Hospital of Philadelphia.

HLA-A2/peptide Tetramer + PE Streptavidin or Brilliant Violet 421™ Streptavidin

Human PBMCs were stained with PerCP anti-human CD8 and labeled with NLVPMVATV-loaded human HLA-A2 tetramer bound to PE Streptavidin, Brilliant Violet 421™ Streptavidin, or Pacific Blue™ Streptavidin. The data shows positive staining with a wide range of tetramer dilutions for PE and BV421™. Data provided by Rick Willis and John Altman, Emory/Yerkes.