Our imaging competition showcased the capabilities of BioLegend microscopy reagents in the hands of our customers. Many beautiful images were submitted, but in the end, we had to select a few well-deserved winners. If you didn’t win this year, keep building your portfolio as new imaging competitions will be here soon. Until then, browse through our winners and other submissions below. For gallery images, you can click on the image to bring up a larger version of the photo.

*Note: While customers may have used the linked products for their submission, the reagents may not have been validated in-house for microscopy applications.

Grand Prize Winner - $500 Amazon gift card

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Hugues Lelouard – Centre d'Immunologie de Marseille-Luminy

Frozen mouse intestinal villi are stained with anti-EpCAM eFluor™ 450 (cyan), purified anti-Meca 32(magenta), anti-Lyve 1 eFluor™ 570 (orange), purified anti-CD11c (red), anti-CX3CR1 GFP (green), anti-UEA-I biotin (grey), purified anti-collagen IV (yellow) and Alexa Fluor® 660 phalloidin (purple). The purified antibodies were detected with goat anti-rabbit Alexa Fluor® 514, donkey anti-armenian hamster Alexa Fluor® 594, donkey anti-rat Alexa Fluor® 647 and Streptavidin Alexa Fluor® 405.

Runner-Up Winners - $100 Amazon gift card

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Ankita Patil – Drexel University

Superior cervical ganglion neurons were stained with anti-tubulin β3 (pink) and anti-TRIM46 (aqua), then stained with secondary antibodies, phalloidin (orange) and DAPI (blue).

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Sebastien Mailfert – Centre d'Immunologie de Marseille-Luminy

A ten-color spectral imaging strategy to reveal localization of gut immune cell subsets in mouse frozen intestine with anti-EpCam (blue), anti-GFP (light green), anti-CD45R (orange), anti-CD11c (brown), anti-CD3 (magenta), anti-UEA-1 lectin(dark green), anti-collagen IV (yellow), Alexa Fluor® 660 Phalloidin (red), Sytox Blue (cyan) and autofluorescence (light gray).


 

Gallery

A single cell from a human KG1 acute myeloid leukemia cell line stained with anti-a4 integrin subunit (green), anti-CD82 (magenta). Imaged using STED super-resolution microscopy where the resolution here is approximately 20 nanometers

Frozen mouse intestinal villi are stained with anti-EpCAM eFluor™ 450 (cyan), purified anti-Meca 32 (magenta), anti-Lyve 1 eFluor™ 570 (orange), purified anti-CD11c (red), anti-CX3CR1 GFP (green), anti-UEA-I biotin (grey), purified anti-collagen IV (yellow) and Alexa Fluor® 660 phalloidin (purple). The purified antibodies were detected with goat anti-rabbit Alexa Fluor® 514, donkey anti-armenian hamster Alexa Fluor® 594, donkey anti-rat Alexa Fluor® 647 and Streptavidin Alexa Fluor® 405.

A ten-color spectral imaging strategy to reveal localization of gut immune cell subsets in mouse frozen intestine with anti-EpCam (blue), anti-GFP (light green), anti-CD45R (orange), anti-CD11c (brown), anti-CD3 (magenta), anti-UEA-1 lectin(dark green), anti-collagen IV (yellow), Alexa Fluor® 660 Phalloidin (red), Sytox Blue (cyan) and autofluorescence (light gray).

Paraffin-embedded neonatal mouse lung sectioned and stained with anti-O/'GlcNac (green), anti-RAGE (red) and DAPI (blue).

Frozen bone marrow sections with osteoclasts expressing tdTomato (yellow) were stained with Flash Phalloidin™ Green 488 (green) and counterstained with DAPI (pink).

Unpassaged live mouse primary retinal pigment epithelial cells engulfed biotinylated photocreceptor outer segments (POS) coupled to Cy5 streptavidin (red).  Cells were subsequently stained with Flash Phalloidin™ Green 488 (green) and DAPI (blue).

Mouse in-vitro cultured proplatelet forming megakaryocytes stained with anti-CD41 Alexa Fluor® 488 (green), Phalloidin Alexa Fluor® 555 (red) and the nucleus stained with DAPI (blue).

Superior cervical ganglion neurons were stained with anti-tubulin β3 (pink) and anti-TRIM46 (aqua), then stained with secondary antibodies, phalloidin (orange) and DAPI (blue).

Frozen human iPSC derived neural rosettes stained with Pax-6 (red) and ZO-1 (green).

Frozen mouse retina stained with anti-PKCα Alexa Fluor® 488 (green), anti-synapsin Alexa Fluor® 555 (red), anti-GFAP Alexa Fluor® 647 (magenta) and DAPI (blue).

Frozen mouse intestine stained with Ankyrin G (green), E-Cadherin (red) and DAPI (blue), followed by anti-goat Alexa Fluor® 488 and anti-rabbit Alexa Fluor® 568.

C57BL/6 inguinal lymph node labeled with anti-CD169-PE (red), single harmonic generation of collagen fiber of LN capsule (blue), CMFDA labelled polyclonal B cells (green).

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