BioLegend Cell Biology and Functional Proteins
BioLegend provides a wide selection of antibodies validated and quality-control tested for a variety of applications including Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), or Chromatin IP (ChIP). Targets include a wide selection of cell biology proteins including cell signaling, cell structure, cell development and differentiation, cell proliferation and growth, cell cycle, epigenetic and transcriptional regulators, cell adhesion, and cell death proteins, as well as epitope tags.
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Research Area
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Western Blot
Direct-Blot™ HRP Antibodies
Western-Ready™ ECL Substrate Kit
Prime Step™ Protein Ladder
Primary Antibodies for Western Blot
Secondary Reagents for Western Blot
Western Blot Controls
Western Blot Sampler Kits
IHC/ICC
Primary Antibodies for IHC
Primary Antibodies for ICC
Secondary Reagents for IHC/ICC
Chemical Probes
 
All Applications
Antibodies for Phospho-Proteins
KO/KD Validated Antibodies
Epitope Tag Antibodies
IP/ChIP
Go-ChIP-Grade™ Products
Antibodies for IP
Affinity Gels for IP/Purification
 
 
Other Applications
Flow Cytometry
ELISA and Multiplex
Cell Function and Viability Assay Kits

Application Protocols
Western Blot
Western Blot Protocol
Direct-Blot™ Protocol
IHC/ICC
IHC Protocol for Frozen Sections
IHC for Paraffin-Embedded Sections
ICC Staining Protocol Kits
ICC for Methanol Fixed Cells
IP/ChIP
Immunoprecipitation Protocol
Chromatin Immunoprecipitation (ChIP) Protocol

BioLegend’s Commitment to Antibody Quality
To ensure the highest quality of products, BioLegend’s cell biology antibodies undergo extensive validation processes, which include:
  • Testing on knockout (KO) or knockdown (KD) cell lysates (CRISPR/Cas9 or siRNA), and negative control cell lines to confirm antibody specificity.
  • Testing on multiple species (human, mouse, rat, yeast, bacteria, zebrafish) whenever applicable, to determine antibody cross-reactivity.
  • Side-by-side comparison testing with competitor’s antibody to ensure sensitivity.
  • Side-by-side comparison testing of lots with internal controls for lot-to-lot consistency.
  • Clones for one application (such as WB or ChIP) further validated in multiple applications (such as microscopy, flow cytometry) to provide the user the flexibility of supplementing their data with additional assays specific to their research.
  • Optimization of antibody suggested use by a titration range.
Learn more about BioLegend's Reproducibility and Validation Initiatives, and how you can improve your own reproducibility.

 
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