Conformation-Specific Antibodies for Alpha-Synuclein
Formation and accumulation of misfolded α-Synuclein aggregates and fibrils is a hallmark of several neurodegenerative disorders known as Synucleinopathies including Parkinson’s disease (PD). Antibodies against this protein are used as the gold standard for immunostaining of Lewy bodies which are a common pathological feature of PD.
To facilitate research in PD, BioLegend has licensed four mouse monoclonal antibodies (clones Syn-O2, Syn-O3, Syn-O4, and Syn-F1) that were generated in the laboratory of Professor Omar El-Agnaf at United Arab Emirates University (UAEU). These clones allow detection of diseased forms of α-Synuclein including those in the intermediate aggregation state. They provide unique specificity for:
  • Aggregated and fibrillar forms of α-Synuclein
  • Minimal cross-reactivity with its native form
  • No cross-reactivity with Amyloid beta (Ab 1-42) or β- and γ-Synucleins
  • Validated in FFPE PD brain tissue sections
  • Suitable for IHC and dot blot
CloneTarget RecognitionFormatApplication
Syn-O2 Syn-O3 Syn-O4Oligomer PreferringPurifiedIHC, Dot Blot
Syn-F1Fibril PreferringPurifiedIHC, Dot Blot
Highlighted relevant publications from Professor El-Agnaf Laboratory
The laboratory has explored the potential use of α-Synuclein as a biomarker for PD. They generated and characterized several novel conformation-specific antibodies for α-Synuclein including clone Syn-O2. They utilized various methods to validate the specificity of this clone in recognizing the oligomeric forms of α-Synuclein. For example, using immunohistochemical studies, they demonstrated staining of Lewy bodies and Lewy neurites with Syn-O2 in tissues derived from PD patients. They then developed a highly specific ELISA assay to quantify α-Synuclein species in biological samples from PD patients and aged-matched healthy controls using clone Syn-O2. Read more in the articles below:

Vaikath NN, et al. 2015. Neurobiol Dis. 79:81 (PubMed)
Majbour NK, et al. 2016. Mol Neurodegener. 11:7 (PubMed)

Download our info sheet on aggregate-specific antibodies for α-Synuclein.

Learn more about Parkinson’s disease and associated proteins.

Featured Antibody
IHC staining of α-Synuclein deposits with anti-α-Synuclein, aggregated antibody (clone Syn-O3) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue.Dot-blot of anti-α-Synuclein antibodies (clones Syn-O3 and 4B12/Synuclein) demonstrating their binding to α-Synuclein conformers (monomeric and fibrillary forms). Note that these antibodies do not cross-react with amyloid beta peptide (Aβ1-42). Clone 4B12/Synuclein was included as a comparison for binding to monomeric and aggregated forms of α-Synuclein.
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