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- Clone
- M1305A02 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD170, Sialic acid-binding Ig-like lectin E, Siglec 9
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Recombinant a-NEu5Ac-PAA (black circles) binds to immobilized recombinant mouse Siglec-E-Fc Chimera (carrier-free) (Cat. No. 551506). Ultra-LEAF™ Purified anti-mouse Siglec-E antibody (purple squares, clone M1305A02) inhibits the binding in a dose-dependent manner. This antibody blocks the binding of 0.8 µg/mL recombinant a-NEu5Ac-PAA protein to 0.5 µg/mL of immobilized recombinant mouse Siglec-E with an ND50 = 0.04 - 0.3 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 934403 | 100 µg | £185 | ||||
| 934404 | 1 mg | £490 | ||||
Siglecs (sialic acid binding Ig-like lectins) are type I membrane proteins with an extracellular region containing a sialic acid binding V-set Ig-like domain at the N-terminus, followed by varying numbers of C2-set Ig domains. The cytoplasmic tails of all siglecs have one or more tyrosines within potential signalling motifs. Siglecs are widely expressed on hematopoietic cells, often in a cell-type-specific manner. Their ligands, sialic acids, are negatively charged monosaccharides found on cell-surface glycoproteins and glycolipids. Studies suggest that siglecs may participate in cell-cell interactions or act as receptors for the entry of viral or bacterial pathogens. In addition, the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domain indicates that these molecules may play a role in the suppression of immunoreceptor signaling. The siglecs can be classified into two subgroups, with Siglec-1, -2, and -4 as one group and a Siglec-3/CD33-related subgroup (Siglec-3, and -5 through -13) as the second. Siglec-E is a mouse CD33-related siglec that selectively regulates early recruitment of neutrophils to the lung in acute lung inflammation induced by lipopolysaccharides. Siglec-E-deficient mice exhibit exaggerated neutrophil recruitment that is reversible by using a blockade of the β2 integrin, CD11b. In addition, sialidase treatment of fibrinogen reverses the suppressive effect of Siglec-E on CD11b signaling, suggesting that sialic acid recognition by Siglec-E is required for its inhibitory function. These findings indicate that Siglec-E is an important negative regulator of neutrophil recruitment to the lung and β2 integrin-dependent signaling.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Mouse Siglec-E
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding between recombinant a-NEu5Ac-PAA and recombinant mouse Siglec-E-Fc chimera (carrier-free) (Cat. No. 551506). The ND50 range is 0.04 - 0.3 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2832884 (BioLegend Cat. No. 934403)
AB_2832885 (BioLegend Cat. No. 934404)
Antigen Details
- Structure
- Homodimer
- Distribution
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Murine Siglec-E is expressed by neutrophils, NK cells, monocytes, macrophages, dendritic cells, and B1 cells.
- Function
- Siglec-E regulates neutrophil recruitment. Siglec-E signaling negatively regulates the LPS-induced production of TNF-α and IL-6 by macrophages. It is upregulated by a variety of TLR agonists.
- Ligand/Receptor
- α2, 8-linked disialic acid
- Cell Type
- Embryonic Stem Cells, Mature Neurons, Neural Stem Cells, Neurons, Schwann Cells
- Biology Area
- Cell Biology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Growth Factors
- Antigen References
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- McMillan SJ, et al. 2013. Blood. 121:2084.
- Bax M, et al. 2010. Ann. Rheum. Dis. 69:42.
- Angata T and Varki A. 2000. J. Biol.Chem. 275:22127.
- Zhang JQ, et al. 2004. Eur. J. Immunol. 34:1175.
- Yu Z, et al. 2001. Biochem. J. 353:483.
- Gene ID
- 83382 View all products for this Gene ID
- UniProt
- View information about Siglec-E on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All Siglec-E Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-mouse Siglec-E | M1305A02 | Block |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Ultra-LEAF™ Purified anti-mouse Siglec-E
Recombinant a-NEu5Ac-PAA (black circles) binds to immobilize...
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