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Purified anti-CD55 Antibody

Pricing & Availability
Clone
M033 (See other available formats)
Regulatory Status
RUO
Other Names
Complement Decay-Accelerating Factor, DAF
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
M033_PURE_CD55_Antibody_1_073119
Western blot of purified anti-CD55 antibody (clone M033). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • M033_PURE_CD55_Antibody_1_073119
    Western blot of purified anti-CD55 antibody (clone M033). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • M033_PURE_CD55_Antibody_2_073119
    IHC staining of purified anti-CD55 antibody (clone M033) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Check Availability Save
868801 25 µg £73
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868802 100 µg £189
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Description

CD55, also known as the complement decay-accelerating factor, is a protein that regulates the complement system on the cell surface. CD55 recognizes the complement C4b and C3b fragments that are generated during C3 and C4 activation, and indirectly blocks the formation of the membrane attack complex. CD55 has also been shown to be a ligand for the seven-span transmembrane receptor CD97. Interaction between CD55 and CD97 has been suggested to play an important role in the pathogenesis of multiple sclerosis and rheumatoid arthritis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When running WB, lysates should be loaded without DTT, and unboiled.

RRID
AB_2814625 (BioLegend Cat. No. 868801)
AB_2814626 (BioLegend Cat. No. 868802)

Antigen Details

Structure
CD55 is a 381 amino acid protein with predicted molecular mass of ~41 kD and observed molecular mass of ~55 kD.
Distribution

Tissue Distribution: Predominantly expressed in the brain, lung, ovary, and placenta
Cellular Distribution: Plasma membrane, extracellular, and Golgi apparatus

Function
CD55 recognizes C4b and C3b fragments that are created during C3 and C4 activation, and indirectly blocks the formation of the membrane attack complex.
Interaction
Forms non-disulfide-linked, covalent homodimer.
Ligand/Receptor
CD97
Cell Type
Endothelial cells
Biology Area
Complement, Innate Immunity, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Blood Group Markers, CD Molecules, Golgi Markers, Innate Immune Signaling
Antigen References
  1. Dho SH, et al. 2018. Immune Netw. 18(1):e11
  2. Nowicki B, et al. 2013. Adv Exp Med Biol. 735:83
  3. Piccoli AK, et al. 2011. Rev Bras Reumatol. 51(5):503
Gene ID
1604 View all products for this Gene ID
UniProt
View information about CD55 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/31/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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