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Ultra-LEAF™ Purified anti-mouse CD268 (BAFF-R) Antibody

Pricing & Availability
Clone
7H22-E16 (See other available formats)
Regulatory Status
RUO
Other Names
BR3, BAFFR, BAFF receptor, TNFRSF13C, B cell-activating factor receptor, BLys receptor 3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
A_7H22-E16_PE_060309
C57BL/6 mouse splenocytes stained with purified 7H22-E16 conjugated to PE (upper) or rat IgG1 PE isotype control (lower panel) and CD45R/B220 APC.
  • A_7H22-E16_PE_060309
    C57BL/6 mouse splenocytes stained with purified 7H22-E16 conjugated to PE (upper) or rat IgG1 PE isotype control (lower panel) and CD45R/B220 APC.
  • B_7H22-E16_PE_060309
  • C_7H22-E16_ULEAF_CD268_Antibody_071519
    Recombinant mouse BAFFR (Black Circles, Cat. No. 756506) binds to immobilized recombinant mouse BAFF (Cat. No. 591202). Anti-mouse BAFFR antibody (Purple Squares, Clone 7H22-E16) inhibits the binding in a dose-dependent manner. This antibody blocks the binding of 0.2 µg/mL recombinant mouse BAFF protein to 1 µg/mL of immobilized recombinant mouse BAFFR with an ND50 = 0.15-1.5 µg/mL.
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134107 100 µg £185
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134108 1 mg £490
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Description

BAFF (B cell-activating factor belonging to the TNF family) receptor (BAFF-R) is one of the three receptors that bind to BAFF. It is a type III transmembrane TNF receptor family member. BAFF-R is expressed at high levels in resting B cells and at lower levels in activated B cells, a small subset of activated/memory CD4+ T cells. It is a major mediator of BAFF dependent co-stimulatory response in peripheral B and T cells. It was reported that the mice expressing defective BAFF-R have disrupted B cell maturation. BAFF receptor appears to be particularly important for the regulation of normal B cell survival and maturation, as well as in the pathophysiology of aggressive B-cell malignancies and autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse BAFF receptor transfected rat RBL cells
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. The antibody blocks the binding between recombinant mouse BAFF (Cat. No. 591202) and recombinant mouse BAFFR (Cat. No. 756506). The ND50 range is 0.15 - 1.5 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Ng LG, et al. 2006. Eur. J. Immunol. 36:1837.
  2. Ng LG, et al. 2004. J Immunol. 173:807.
  3. Batten M, et al. 2000. J. Exp. Med. 192:1453.
  4. Fu L, et al. 2009. Blood 113:4627.
RRID
AB_2814029 (BioLegend Cat. No. 134107)
AB_2876470 (BioLegend Cat. No. 134108)

Antigen Details

Structure
A type III transmembrane TNF receptor family member
Distribution

Expressed on most B cells, a small subset of CD4+ T cells.

Function
Play an importance role in B cell development, maturation, and survival.
Ligand/Receptor
BAFF (BLyS)
Cell Type
B cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Thompson JS, et al. 2001. Science 293:2108.
2. Schiemann B, et al. 2001. Science 293:2111.
3. Batten M, et al. 2000. J. Exp. Med. 192:1453.
4. Fu L, et al. 2009. Blood 113:4627.

Gene ID
72049 View all products for this Gene ID
UniProt
View information about CD268 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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