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Purified anti-SMN (Gemin 1) Antibody

Pricing & Availability
Clone
P85H1 (See other available formats)
Regulatory Status
RUO
Other Names
SMN1, SMN, SMN2, SMNT, GEMIN1, Survival of Motor Neuron 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P85H1_PURE_SMN_Antibody_062119
Western blot of purified anti-SMN (Gemin 1) antibody (clone P85H1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • P85H1_PURE_SMN_Antibody_062119
    Western blot of purified anti-SMN (Gemin 1) antibody (clone P85H1). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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867501 25 µg £73
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867502 100 µg £189
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Description

SMN1/Gem-associated protein 1 (Gemin 1) is a component of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. The SMN complex localizes to the cytosol and nucleus of eukaryotic cells. SMN1 gene is nearly identical to the SMN2 gene, and both SMN1 and SMN2 encode the SMN protein. Mutations of SMN1 lead to spinal muscular atrophy.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant fragment of human SMN1 (amino acids 148-294) expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810803 (BioLegend Cat. No. 867501)
AB_2810804 (BioLegend Cat. No. 867502)

Antigen Details

Structure
SMN1 is a 294 amino acid protein with a predicted molecular mass of ~ 32 kD and an observed molecular mass at ~ 35 kD.
Distribution

Tissue Distribution: Brain, pancreas, gallbladder, bladder, testis, lymph node, and bone marrow
Cellular Distribution: Nucleus and cytosol

Function
SMN1 is a component of the SMN complex essential for the formation of snRNP complexes in Eukaryotic cells.
Interaction
SMN1 forms homodimer or found in complex with other SMN/GEMIN proteins.
Cell Type
Neurons
Biology Area
Chromatin Remodeling/Epigenetics, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family
Enzymes and Regulators
Antigen References
  1. Maretina MA, et al. 2018. Curr. Genomics. 19(5):339-55.
  2. Singh RN, Singh NN. 2018. Adv. Neurobiol. 20:31-61.
  3. Groen EJN, et al. 2018. Nat. Rev. Neurol. 14:214-24.
Gene ID
6606 View all products for this Gene ID 6607 View all products for this Gene ID
UniProt
View information about SMN on UniProt.org
Go To Top Version: 1    Revision Date: 06/21/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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