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Purified anti-PRKD2 Antibody

Pricing & Availability
Clone
O95G1 (See other available formats)
Regulatory Status
RUO
Other Names
Protein Kinase D2, Serine/Threonine-Protein Kinase D2, NPKC-D2, PKD2
Isotype
Mouse IgG2b
Ave. Rating
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Product Citations
publications
O95G1_Purified_PRKD2_Antibody_1_052219.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PRKD2 Antibody, clone O95G1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • O95G1_Purified_PRKD2_Antibody_1_052219.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-PRKD2 Antibody, clone O95G1, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • O95G1_Purified_PRKD2_Antibody_2_052219.png
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:500 dilution (1.0 µg/mL) of either Purified Mouse IgG2b isotype control Antibody (Cat. No. 402202, panel A) or Purified anti-PRKD2 Antibody, Clone O95G1 (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
  • O95G1_Purified_PRKD2_Antibody_3_052219.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of Purified Mouse IgG2b isotype control Antibody (Cat. No. 402202) or Purified anti-PRKD2 Antibody, clone O95G1. The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a rabbit control antibody against a separate epitope of PRKD2. Lane M: Molecular Weight marker.
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617301 25 µg £70
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617302 100 µg £174
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Description

Protein Kinase D2 (PRKD2) is one of 3 members of the Protein Kinase D family of calcium-calmodulin dependent serine/threonine kinases. PRKD2 is a downstream regulator of protein kinase C family members and can be directly activated by diacylglycerol. PRKD2 regulates transport vesicle fission from the trans-golgi network. PRKD2 deficiency promotes β-cell insulin secretion and concomitant hyperinsulinemia and metabolic disorders, and loss of PRKD2 results in impaired dense granule secretion in platelets. Moreover, PRKD2 has been shown to fuel uncontrolled growth and invasion in many types of cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human PRKD2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/ml. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone does not recognize mouse PRKD2.

While this clone is able to immunoprecipitate PRKD2 from whole cell extracts, the resulting fraction was not significantly enriched in PRKD2. Therefore, this clone may not be suitable for detecting transient or low-abundance protein complexes by co-IP.

RRID
AB_2810667 (BioLegend Cat. No. 617301)
AB_2810668 (BioLegend Cat. No. 617302)

Antigen Details

Structure
PRKD2 is an 878 aminoacid protein with a predicted molecular weight of 97 kD.
Distribution

Cytosolic, nuclear, and trans-golgi network (TGN)/Ubiquitously expressed

Function
Cell signaling, TGN-mediated trafficking
Biology Area
Protein Trafficking and Clearance, Signal Transduction
Molecular Family
Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Xiao Y, et al. 2018. Nat. Commun. 9: 2015.
  2. Konopatskaya O, et al. 2011. Blood. 118:416.
  3. Yeaman C, et al. 2004. Nat. Cell Biol. 6: 106.
  4. Sturany S, et al. 2001. J. Biol. Chem. 276: 3310.
Gene ID
25865 View all products for this Gene ID
UniProt
View information about PRKD2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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