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Purified anti-Catalase Antibody

Pricing & Availability
Clone
Poly28607 (See other available formats)
Regulatory Status
RUO
Other Names
CAT, Epididymis Secretory Sperm Binding Protein
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly28607_Purified_Catalase_Antibody_1_051319.png
Western blot of purified anti-Catalase antibody (Poly28607). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28607_Purified_Catalase_Antibody_1_051319.png
    Western blot of purified anti-Catalase antibody (Poly28607). Lane 1: Molecular weight marker; Lane 2: 5 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28607_Purified_Catalase_Antibody_2_051319.png
    IHC staining of purified anti-Catalase antibody (Poly28607) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28607_Purified_Catalase_Antibody_3_051319.png
    IHC staining of purified anti-Catalase antibody (Poly28607) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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860701 25 µg £86
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Description

Catalase is a heme-containing homotetrameric protein that decomposes hydrogen peroxide to water and oxygen to prevent the generation of hydroxyl radical in cells. The role of catalase in cellular oxidative stress defense has been widely studied. Overexpression of catalase makes cells more resistant to  hydrogen peroxide-induced toxicity and oxidant-mediated injury from hypoxia. Transgenic mice overexpressing catalase are protected against myocardial injury following adriamycin treatment. Although knockout mice of catalase develop normally, they show differential sensitivity to oxidant tissue injury. Brain is particularly vulnerable to oxidative stress. Abnormalities and dysfunction of catalase have been indicated in Parkinson’s disease, Alzheimer’s disease and others. Data show that in parkinsonian brain, catalase activity is reduced in the substantia nigra and putamen. In vitro cellular models show aggregated Abeta has high affinity for catalase, which leads to the accumulation of hydrogen peroxide and increased oxidative stress.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Catalase (Ala401-Glu523) with two N-terminal Tags, His-tag and S-tag expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810741 (BioLegend Cat. No. 860701)

Antigen Details

Structure
Human Catalase is a 527 amino acid protein with a molecular mass of 60 kD.
Distribution

Tissue distribution: Ubiquitously expressed; highly expressed in bone marrow and liver.
Cellular distribution: Peroxisome and nucleus

Function
Catalase is an antioxidant enzyme involved in the body defense against oxidative stress.
Cell Targets
Hydrogen peroxide
Cell Type
Astrocytes, Neurons
Biology Area
Cell Biology, Cell Death, Neurodegeneration, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Ho YS, et al. 2004. J Biol Chem. 279(31):32804-12.
  2. Ambani LM, et al. 1975. Arch Neurol. 32(2):114-8.
  3. Milton NG. 1999. Biochem J. 344(Pt 2): 293–296.
  4. Habib LK, et al. 2010. J Biol Chem.  285(50):38933-43.
Gene ID
847 View all products for this Gene ID
UniProt
View information about Catalase on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/13/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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