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Purified anti-SMAD1 Phospho (S463/S465)/SMAD9 (S465/S467) Antibody

Pricing & Availability
Clone
A17019A (See other available formats)
Regulatory Status
RUO
Other Names
Mothers against decapentaplegic homolog 1, JV4-1, Mad-related protein 1
Isotype
Mouse IgG1, λ
Ave. Rating
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Product Citations
publications
A17019A_Purified_SMAD1-PhosphoslashSMAD9-Phospho_Antibody_021919.png
Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells untreated (-) or treated (+) with 100 ng/mL BMP-4 (Cat. No. 595202 and 595302) for 45 minutes were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of purified anti-SMAD1 Phospho (Ser463/Ser465)/SMAD9 Phospho (Ser465/Ser467), clone A17019A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405306) at a 1:3000 dilution. Equal SMAD1 loading was confirmed by probing membranes with an anti-pan SMAD1 antibody. Lane M: Molecular Weight Marker.
  • A17019A_Purified_SMAD1-PhosphoslashSMAD9-Phospho_Antibody_021919.png
    Whole cell extracts (15 µg protein) from serum starved HeLa and NIH/3T3 cells untreated (-) or treated (+) with 100 ng/mL BMP-4 (Cat. No. 595202 and 595302) for 45 minutes were resolved on a 4-12% Bis-Tris gel, transferred to nitrocellulose and probed with 1.0 µg/mL (1:500 dilution) of purified anti-SMAD1 Phospho (Ser463/Ser465)/SMAD9 Phospho (Ser465/Ser467), clone A17019A, for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405306) at a 1:3000 dilution. Equal SMAD1 loading was confirmed by probing membranes with an anti-pan SMAD1 antibody. Lane M: Molecular Weight Marker.
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610401 25 µg £77
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610402 100 µg £182
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Description

SMAD1 and SMAD9 (SMAD8) are members of SMAD family, whose members play a critical role in signal transduction from the TGF-β receptor kinases. Activation of these receptors results in phosphorylation of SMAD1 and SMAD9 at a C-terminal SSXS motif (Ser463/Ser465 in SMAD1; Ser465/Ser467 in SMAD9), leading to dimerization with the coactivator SMAD4. The SMAD1-4 complex is translocated to the nucleus where it induces expression of target genes involved in differentiation, self-renewal, proliferation, and apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Because the sequence of the synthetic phosphopeptide immunogen is completely conserved between SMAD1 and the corresponding residues in SMAD9 (Ser465/Ser467), this clone is predicted to recognize both phosphorylated proteins.

This clone is predicted to recognize rat SMAD1 Phospho (Ser463/Ser465) and SMAD9 Phospho (Ser465/Ser467) due to complete sequence homology between the immunizing peptide and each rat homolog.

RRID
AB_2801107 (BioLegend Cat. No. 610401)
AB_2801108 (BioLegend Cat. No. 610402)

Antigen Details

Structure
SMAD1 is a 465 amino acid protein with a predicted molecular weight of 52 kD. SMAD9 is 467 amino acid protein with a predicted molecular weight of 52 kD.
Distribution

Ubiquitous expression/cytosolic and nuclear distribution

Function
Transcriptional activation/TGF- β signaling
Interaction
SMAD4, SMURF1, USP15, CBP, p300
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability, Signal Transduction, Transcription Factors
Molecular Family
Phospho-Proteins
Antigen References
  1. Hoodless PA, et al. 1996. Cell. 85: 489-500.
  2. Whitman M. 1998. Genes Dev. 12: 2445-62.
  3. Alarcon C, et al. 2009. Cell. 139: 757-69.

 

Gene ID
4086 View all products for this Gene ID 4093 View all products for this Gene ID
UniProt
View information about SMAD1 Phospho Ser463/Ser465 SMAD9 Phospho Ser465/Ser467 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/19/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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