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- Regulatory Status
- RUO
- Other Names
- GDNF family receptor alpha-3, GDNF receptor alpha-3, GDNFR-alpha-3, GFR-alpha-3, GFRA3, Glial cell line-derived neurotrophic factor receptor alpha-3
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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When human Artemin is immobilized at 1.0 µg/mL, human GFR alpha-3-Fc Chimera binds with EC50 of 20 - 80 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding. -
Stability testing for Human GFR alpha-3. Human GFR alpha-3 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4°C (Control). The samples were tested for their ability to bind human Artemin.
Glial cell line derived neurotrophic factor (GDNF) family receptor alpha 3 (GFRA3, GDNFRa3) is a member of GDNF receptor family. These receptors are glycosylphosphoinositol (GPI)-anchored cell surface receptors containing 3 conserved cysteine repeats and mediate the activation of the RET tyrosine kinase receptor. GFRA3 is expressed in the central nervous system during the earliest stages of neurogenesis, and later it is predominantly found in developing and adult nociceptive sensory neurons. GFRA3 is a potent survival factor for central and peripheral neurons. Its ligand artemin is primarily expressed by immature Schwann cells in the peripheral nervous system, and vascular smooth muscle cells, directing axonal projection of sympathetic neurons. GFRA3 mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. Artemin first binds GFRA3 and recruits RET, forming a signaling complex that is a pentamer containing one artemin, two GFRA3, and two RET molecules. The RET is the receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.
Product DetailsProduct Details
- Source
- Human GFR alpha-3/GFRA3, amino acid Asp32-Asn374 (Accession # O60609) with a C-terminal human IgG Fc tag was expressed in CHO cells.
- Molecular Mass
- The 582 amino acid recombinant protein has a predicted molecular mass of approximately 65.2 kD. The DTT-reduced proteins migrate at approximately 75 kD, and non-reduced proteins migrate at approximately 150 kD by SDS-PAGE. The predicted N-terminal amino acid is Asp.
- Purity
- > 95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When human Artemin is immobilized at 1.0 µg/mL, human GFR alpha-3-Fc Chimera binds with EC50 of 20 - 80 ng/mL. HRP Protein A (Cat. No. 689202) was used to detect the binding.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide linked homodimer
- Function
- Receptor for the glial cell line-derived neurotrophic factor, Artemin/ARTN.
- Ligand/Receptor
- Artemin/ARTN
- Bioactivity
- Measured by its ability to bind human Artemin
- Cell Sources
- Widely expressed in adult and fetus which exhibit a similar pattern.
- Cell Type
- Neural Stem Cells, Neurons
- Biology Area
- Cell Biology, Neuroscience, Stem Cells
- Molecular Family
- Soluble Receptors
- Antigen References
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- Baloh RH, et al. 1998. Proc. Natl. Acad. Sci. USA 95:5801-6.
- Baloh RH, et al. 1998. Neuron 21:1291-302.
- Nishino J, et al. 1999. Neuron 23:725-36.
- Honma Y, et al. 2002. Neuron 35:267-82.
- Veiga-Fernandes H, et al. 2007. Nature 446:547-51.
- Gene ID
- 2676 View all products for this Gene ID
- UniProt
- View information about GFRalpha3 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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