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- Clone
- 3D6C02 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells were treated with (filled histogram) and without (dashed histogram) Cell Activation Cocktail for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, then intracellulary stained with anti-IκB-α (clone 3D6C02) PE and anti-CD3 (UCHT1) APC. Histograms were gated off of CD3+ lymphocytes. -
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were treated with (filled histogram) and without (dahed histogram) 100 ng/mL LPS for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, then intracellulary stained with anti-IκB-α (clone 3D6C02) PE.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 662411 | 25 tests | £113 | ||||
| 662412 | 100 tests | £255 | ||||
IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, Hamster
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length human IκB-α recombinant protein expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.0075 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against I?B-a.
- Product Citations
-
- RRID
-
AB_2783365 (BioLegend Cat. No. 662411)
AB_2783366 (BioLegend Cat. No. 662412)
Antigen Details
- Structure
- Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
- Distribution
-
Cytoplasm
- Function
- Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
- Interaction
- NFkB1, SUMO4, p53, ribosomal S6 kinase 1
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
- Molecular Family
- Tumor Suppressors
- Antigen References
-
1. Baeuerle PA. 1998. Cell 95:729.
2. Hoffmann A, et al. 2002. Science 298:1241.
3. Ito CY, et al. 1995. Genomics 29:490. - Gene ID
- 4792 View all products for this Gene ID
- UniProt
- View information about IkappaB-alpha on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All IκB-α Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-IκB-α | 3D6C02 | WB,ICC |
| Brilliant Violet 421™ anti-IκB-α | 3D6C02 | ICC |
| PE anti-IκB-α | 3D6C02 | ICFC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-IκB-α
Western blot analysis of cell lysates from HeLa (Human), Raw... 
HeLa cells were stained with purified anti-IκBα (clone 3D6C0... 
Hela cells were fixed with cold methanol at -20°C for 15 min... 
Total lysates (15 µg protein) from HepG2 (Human) and CHO (Ha... -
Brilliant Violet 421™ anti-IκB-α
HeLa cells were fixed with cold 100% methanol for 10 minute... -
PE anti-IκB-α
Human peripheral blood mononuclear cells were treated with (... 
Thioglycolate-elicited BALB/c mouse peritoneal macrophages w...
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