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Purified anti-Dopamine Receptor D1 Antibody

Pricing & Availability
Clone
A17050E (See other available formats)
Regulatory Status
RUO
Other Names
DRD1, Dopamine Receptor D1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A17050E_Pure_Dopamine-Receptor-D1_Antibody_1_110818
Western blot of purified anti-Dopamine Receptor D1 antibody (clone A17050E). Lane 1: Molecular weight marker; Lane 2: 40 µg of mouse brain lysate; Lane 3: 40 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_1_110818
    Western blot of purified anti-Dopamine Receptor D1 antibody (clone A17050E). Lane 1: Molecular weight marker; Lane 2: 40 µg of mouse brain lysate; Lane 3: 40 µg of rat brain lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_2_110818
    Western blot of purified anti-Dopamine Receptor D1 antibody (clone A17050E). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain lysate membrane fraction; Lane 3: 40 µg of human brain total lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_3_110818
    IHC staining of purified anti-Dopamine Receptor D1 antibody (clone A17050E) on formalin-fixed paraffin-embedded human Substantia Nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_4_110818
    IHC staining of purified anti-Dopamine Receptor D1 antibody (clone A17050E) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • A17050E_Pure_Dopamine-Receptor-D1_Antibody_5_110818
    IHC staining of purified anti-Dopamine Receptor D1 antibody (clone A17050E) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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856001 25 µg £73
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856002 100 µg £189
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Description

The D1 subtype of the dopamine receptor (DRD1) is a Gs/a-protein coupled receptor mainly expressed in the caudate putamen of the central nervous system. Upon binding to its ligand, DRD1 indirectly activates cyclic AMP-dependent protein kinase and regulates neuronal development. Two transcript variants, DRD1a and DRD1b exist in vivo. DRD1 has been discovered to modulate DRD2-mediated signaling pathway. DRD1 and DRD2 also form heterodimers via C-terminal regions, which has been reported to be involved in phospholipase C and CaMKII activation, and BDNF production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human DRD1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783448 (BioLegend Cat. No. 856001)
AB_2783449 (BioLegend Cat. No. 856002)

Antigen Details

Structure
DRD1 is a 446 amino acid protein with an apparent molecular mass of ~ 49 kD.
Distribution

Tissue Distribution: Mainly expressed in the midbrain.
Cellular Distribution: Plasma membrane.

Function
DRD1 is involved in dopamine related signaling and neuronal development.
Interaction
Interacts with DRD2, DNAJC14, and DRD1IP.
Ligand/Receptor
Receptor of Dopamine
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Adhesion Molecules, GPCR
Antigen References
  1. Amare AT, et al. 2017. EPMA J. 8(3):211
  2. Arango-Lievano M, et al. 2015. Med Sci (Paris). 31(5):546
  3. Serretti A, et al. 2007. J Alzheimers Dis. 12(1):73
Gene ID
1812 View all products for this Gene ID 13488 View all products for this Gene ID
UniProt
View information about Dopamine Receptor D1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/08/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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