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PerCP/Cyanine5.5 anti-human CD355 (CRTAM) Antibody

Pricing & Availability
Clone
Cr24.1 (See other available formats)
Regulatory Status
RUO
Other Names
Class I-MHC-Restricted T-cell Associated Molecule
Isotype
Mouse IgG2a, κ
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Product Citations
publications
Cr24dot1_PerCPCyanine55_CD355_Antibody_110618
PMA/Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were stained with CD8a (clone RPA-T8) APC and CD355 (clone Cr24.1) PerCP/Cyanine5.5 (left) or Mouse IgG2a, ? PerCP/Cyanine5.5 Isotype Control (right).
  • Cr24dot1_PerCPCyanine55_CD355_Antibody_110618
    PMA/Ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were stained with CD8a (clone RPA-T8) APC and CD355 (clone Cr24.1) PerCP/Cyanine5.5 (left) or Mouse IgG2a, ? PerCP/Cyanine5.5 Isotype Control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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339111 25 tests £121
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339112 100 tests £282
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Description

CRTAM, (class-I MHC-restricted T-cell associated molecule), is homodimer protein belonging to Ig superfamily. It is expressed transiently on activated NK cells,NKT cells and a subset of CD8+ T cells. Nectin-like molecule-2 (Necl2, TSCL1) is the ligand of CRTAM. CRTAM plays an important role in cell adhesion and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CRTAM-P815 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kent S, et al. 2005. Blood. 106:779
RRID
AB_2783221 (BioLegend Cat. No. 339111)
AB_2783222 (BioLegend Cat. No. 339112)

Antigen Details

Structure
Dimer, Ig superfamily
Distribution

Activated NK cells and subset of CD8+ cells

Function
Adhesion and migration
Ligand/Receptor
Nectin-like molecule 2 (Necl2)
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, MHC Antigens
Antigen References

1. Arase N, et al. 2005. Intl. Immunol. 17:1227
2. Kennedy J, et al. 2000. J. Leukoc. Biol. 67:725

Gene ID
56253 View all products for this Gene ID
UniProt
View information about CD355 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 11/06/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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