Login / Register 
United States ($) USD
Austria (€) EUR
Belgium (€) EUR
Finland (€) EUR
France (€) EUR
Germany (€) EUR
Ireland (€) EUR
Japan (¥) JPY
Luxembourg (€) EUR
Netherlands (€) EUR
Switzerland (CHF) CHF
United Kingdom (£) GBP
- Regulatory Status
- RUO
- Other Names
- FZD10, CD350, Fz-10
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human Frizzled-10-Fc inhibits Topflash reporter activity induced by 50 ng/mL of mouse Wnt3a (Cat. No. 772301) in a dose dependent manner. ED50 for this effect is 0.5 – 2.0 µg/mL. -
Stability testing for Human Frizzled-10. Human Frizzled-10 was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was freeze and thawed four times (4x freeze/thaws), and compared to a control kept at 4⁰C (control). The samples were tested for their ability to inhibit Topflash reporter activity induced by 100 ng/mL of mouse Wnt3a (Cat. No. 772301).
Frizzled-10 (FZD10), also known as CD350, is a G-protein coupled receptor with seven transmembrane domains. It is one of ten Frizzled family of Wnt receptors whose extracellular cysteine rich domain (CRD) binds Wnt proteins. Frizzled receptors are ubiquitously and dynamically expressed in most cells. Frizzled receptors associate with low density lipoprotein receptor-related proteins (LRP6/LRP6) to transduce Wnt signals through the activation of disheveled proteins, inhibition of GSK-3 kinase, and stabilization of beta-catenin which accumulates in the nucleus to promote Wnt-mediated gene transcriptions. Another signaling pathway involving PKC and calcium fluxes has been described, but it is not yet established as a distinct pathway independent of canonical pathway. Frizzled-10 is highly expressed in the placenta, fetal kidney, lung and brain. In the adult brain, it is expressed in the cerebellum, cerebral cortex, medulla and spinal cord. Weak expression is also detected in adult heart, lung, skeletal muscle, pancreas, spleen and prostate. Frizzled-10 is also up-regulated in several cancers and transformed cell lines. It may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and differentiation.
Product DetailsProduct Details
- Source
- Human Frizzled-10, amino acids Ile21-Gly161 (Accession # NM_007197.3) with a C-terminal human IgG1 (Pro100-Lys330) Fc tag was expressed in 293E cells.
- Molecular Mass
- The 380 amino acid recombinant protein has a predicted molecular mass of approximately 43 kD. The DTT-reduced protein migrates at approximately 50 kD and and non-reduced protein migrates at approximately 100 kD by SDS-PAGE. The predicted N-terminal amino acid is Ile.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS, pH 7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- ED50= 0.5 – 2.0 µg/mL as measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells induced by 50 ng/mL of recombinant Wnt3a (Cat. No. 772301).
- Application
-
Bioassay
- Application Notes
-
BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
-
Expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney.
- Function
- Receptor for Wnt proteins that functions in the canonical Wnt/beta-catenin signaling pathway
- Ligand/Receptor
- Wnt7a, Wnt7b, Wnt1, Wnt3a
- Bioactivity
- Measured by its ability to inhibit Wnt3a activity in Topflash assay.
- Biology Area
- Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure
- Molecular Family
- Soluble Receptors
- Antigen References
-
- Koike J, et al. 1999. Biochem Biophys Res Commun. 262:39-43
- Kawakami Y, et al. 2000. Mech Dev. 91:375-378
- Dann CE, et al. 2001. Nature. 412:86-90
- Nagayama S, et al. 2005. Oncogene. 24:6201-6212
- Kwon HS, et al. 2009. Mol Cell Biol. 29:2139-2154
- Galli LM, et al. 2014. Dev Dyn. 243:833-843
- Gene ID
- 11211 View all products for this Gene ID
- UniProt
- View information about Frizzled-10 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
-
• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
-
We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
-
The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
-
Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
-
Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
-
Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
Follow Us