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Purified anti-PAK2 Antibody

Pricing & Availability
Clone
P83B5/PAK2 (See other available formats)
Regulatory Status
RUO
Other Names
p21-activated kinase 2
Isotype
Mouse IgG1
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Product Citations
publications
P83B5slashPAK2_Pure_PAK2_Antibody_1_051818
Western blot of purified anti-PAK2 antibody (clone P83B5/PAK2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of human brain nuclear lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of mouse brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). β-tubulin (Cat. No. 801206) was used as loading control. Enhanced chemiluminescence was used as the detection system.
  • P83B5slashPAK2_Pure_PAK2_Antibody_1_051818
    Western blot of purified anti-PAK2 antibody (clone P83B5/PAK2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of human brain nuclear lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of mouse brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). β-tubulin (Cat. No. 801206) was used as loading control. Enhanced chemiluminescence was used as the detection system.
  • P83B5slashPAK2_Pure_PAK2_Antibody_2_051818
    Western blot of purified anti-PAK2 antibody (clone P83B5/PAK2). Lane 1: Molecular weight marker; Lane 2: 20 µg of Hela cell lysate; Lane 3: 20 µg of SH-SY5Y cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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853401 25 µg £77
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853402 100 µg £189
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Description

PAK2 (p21-activated kinase 2) is a serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, and proliferation. It acts as the downstream effector of the small GTPases CDC42 and RAC1. PAK2 stimulates cell survival and cell growth, and knockdown of PAK2 induces cell cycle arrest. PAK2 is also implicated in the immune system. Loss of PAK2 results in reduced expression of multiple Treg functional molecules, including Foxp3, CD25, Nrp-1 and CTLA-4, coupled with a loss of Treg suppressive function in vitro and in vivo. The p21-activated kinases (PAKs) are the main effectors for the small Rho GTPases, critically involved in neurodevelopment, plasticity and maturation of the nervous system.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment of human PAK2 (a.a. 4-223) protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody specifically recognizes human PAK2 and does not cross-react with mouse or rat PAK2.

RRID
AB_2734648 (BioLegend Cat. No. 853401)
AB_2734649 (BioLegend Cat. No. 853402)

Antigen Details

Structure
PAK2 is a 524 amino acid protein with a molecular mass of 58 kD.
Distribution

Tissue distribution: PAK2 is ubiquitously expressed. Higher expression levels of PAK2 are observed in skeletal muscle, ovary, thymus and spleen.
Cellular distribution: Nucleus.

Function
PAK2 is a serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis, and proliferation.
Interaction
PAK2 interacts with CDC42/p21, RAC1, and SH3MD4.
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance, Stem Cells
Molecular Family
Enzymes and Regulators, Protein Kinases/Phosphatase
Antigen References
  1. Fuchsova B, et al. 2016. Neuroscience. 333:331
  2. O’Hagan KL, et al. 2017. Sci Rep. 7:17097
  3. Koo KH and Kwon H. 2018. Cell Death Dis. 9:77
Gene ID
5062 View all products for this Gene ID
UniProt
View information about PAK2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/21/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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