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Purified anti-mouse Keratin 6A Antibody (Previously Covance catalog# PRB-169P)

Pricing & Availability
Clone
Poly19057 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin, type II cytoskeletal 6A, K6A, CK-6A, mK6-alpha, keratin-6A, 60-kD keratin, cytokeratin-6A, keratin-6-alpha
Previously
Covance Catalog# PRB-169P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
A_Poly19057_PURE_Keratin6A_IHC_021715
Staining of Mouse Keratin 6, on mouse tongue at a 1:5000 dilution.
  • A_Poly19057_PURE_Keratin6A_IHC_021715
    Staining of Mouse Keratin 6, on mouse tongue at a 1:5000 dilution.
  • B_Poly19057_PURE_Keratin6A_WB_050118
    Total lysates (15 µg protein) from 293E transfected with or without mouse keratin 6A were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (1 ug/ml) purified anti-mouse keratin 6A antibody (clone Poly19057). Proteins were visualized using chemiluminescence detection by incubation with and HRP goat anti-mouse IgG secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • C_Poly19057_PURE_Keratin6A_IHC_2_031521
    IHC staining of purified anti-mouse Keratin 6A antibody (Poly19057) on formalin-fixed paraffin-embedded mouse esophagus tissue. Following antigen retrieval using Citrate (Cat. No. 420901), the tissue was incubated with 1.0 µg/mL of the primary antibody 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, AEC, Cat. No. 929501) was used for detection followed by hemotoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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905702 25 µL £61
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905701 100 µL £197
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Description

Keratin 6A is a type II cytokeratin that belongs to the keratin family of fibrous structural proteins. The protein is expressed along with its partners KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. The murine genome is known to have two keratin 6 (CK6) genes, CK6A and CK6b. Mutations in these genes have been associated with pachyonychia congenita.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 6 protein.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
• IF: 1:500
• IHC: 1:500

Application Notes

This antibody is effective in immunoblotting (WB), immunofluorescence (IF) and immunohistochemistry (IHC). This antibody has been successfully used in both frozen and paraffin embedded tissues.

*Predicted MW = 59.9 kD


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Welm AL, et al. 2005. Proc Natl Acad Sci USA. 102:4324.
  2. Hu Y, et al. 2001. Nature. 410:710.
  3. Yuspa SH, et al.. 1989. J Cell Biol. 109:1207.
  4. Roop DR, et al. 1984. J Biol Chem. 259:8037.
  5. Easter SL, et al. 2014. PLoS One. 9:113247. (IF, WB) PubMed
  6. DiTommaso T, et al. 2014. PLoS Genet. 10:1004706. (IHC) PubMed
  7. Li Z, et al. 2007. Cancer Cell. 12:542. (IHC)
  8. Liang Y, 2011 Patholog Res Int. 2011:936794. (IHC) PubMed
  9. Frew IJ, et al. 2008. Mol Cell Biol.28:4536. (IHC) PubMed
  10. Smith BA, et al. 2012. Genes Cancer.3:550. (IHC) PubMed
  11. Johnson JP, et al. 2014 Cancer Geomics Proteomics. 11:115. (IHC) PubMed
  12. Sengupta A, et al. 2010. PLoS One. 5:12249. (IHC) PubMed
  13. Mikaelian I, et al. 2006. Vet Pathol. 43:36. (IHC)
  14. Li W, et al. 2007. J Biol Chem. 282:1161. (IHC, WB) PubMed
  15. Osmanagic-Myers S, et al.2006. J Cell Biol. 174:557. (WB) PubMed
  16. Kittrell FS, et al. 2011. Breast Cancer Res.13:41. (IF) PubMed
  17. Quante T, et al. 2014. Front Oncol. 26:168. (IF) PubMed
  18. Bayo P, Sanchis A, Bravo A, Cascallana JL, Buder K, Tuckermann J, Schütz G, and Pérez P. Glucocorticoid Receptor Is Required for Skin Barrier Competence. Endocrinology, 149: 1377-1388, Mar 2008. (IHC) PubMed
Product Citations
  1. Murai K, et al. 2022. Nat Commun. 13:6206. PubMed
  2. Sengupta A, et al. 2010. PLoS One. 5:e12249. PubMed
  3. Kashgari G, et al. 2020. Developmental Cell. 52(6):764-778. PubMed
  4. Avigad Laron E, et al. 2018. Cell Rep. 24:909. PubMed
  5. Whiteley J, et al. 2018. Stem Cells Transl Med. 7:591. PubMed
  6. Badolati N, et al. 2018. Nutrients. 10:. PubMed
  7. Sweat YY, et al. 2020. J Dent Res. 99:1397. PubMed
  8. Altshuler A, et al. 2021. Cell Stem Cell. 28(7):1248-1261.e8. PubMed
  9. Laurien L, et al. 2020. Nat Commun. 11:1747. PubMed
  10. Sarper SE, et al. 2019. Dis Model Mech. 12. PubMed
  11. Schünke H, et al. 2021. Nat Commun. 12:5912. PubMed
  12. Bornes L, et al. 2021. Life Sci Alliance. 4:00. PubMed
  13. Chen S, et al. 2015. Sci Rep. 5: 18104. PubMed
  14. Xu M, et al. 2019. Am J Transl Res. 11:257. PubMed
  15. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  16. Jiao H, et al. 2020. Nature. 580:391. PubMed
  17. Xu KK, et al. 2020. Invest Ophthalmol Vis Sci. 61:30:00. PubMed
  18. Altmeier S, et al. 2016. PLoS Pathog. 12: 1005882. PubMed
  19. Rogerson C, et al. 2018. Biochim Biophys Acta. 37:2022. PubMed
  20. Weber MS, et al. 2021. Elife. 10: . PubMed
  21. Carceller E, et al. 2016. Sci Rep. 6:38825. PubMed
  22. Dedeić Z, et al. 2018. Cell Death Differ. 25:1289. PubMed
  23. Seldin L, et al. 2020. Developmental Cell. 55(5):558-573.e6. PubMed
  24. Turpin J, et al. 2016. Oncogene. 10.1038/onc.2016.129. PubMed
  25. Osmanagic-Myers S, et al. 2006. J Cell Biol. 174:557-568. PubMed
  26. Bayo P, et al. 2008. Endocrinology. 149:1377-1388. PubMed
  27. Fang X, et al. 2019. Am J Pathol. 189:1212. PubMed
  28. Liang Y 2011. Patholog Res Int. 2011:936794. PubMed
  29. Kittrell F, et al. 2011. Breast Cancer Res. 13:R41. PubMed
  30. Easter S, et al. 2014. PLoS One. 9:e113247. PubMed
  31. Jee JP, et al. 2019. Int J Nanomedicine. 14:5449. PubMed
  32. Reehorst CM, et al. 2021. Development. 148:. PubMed
  33. Buonanno M, et al. 2016. PLoS One. 11: 0138418. PubMed
  34. Li W, et al. 2007. J Biol Chem. 282:1161-1169. PubMed
  35. Kousa YA, et al. 2017. Dev Dyn. 246:670. PubMed
  36. Quante T, et al. 2014. Front Oncol. 4:168. PubMed
  37. Frew I, et al. 2008. Mol Cell Biol. 28:4536-4548. PubMed
  38. Crompton R, et al. 2016. Lab Invest. 10.1038/labinvest.2015.160. PubMed
  39. Shatirishvili M, et al. 2016. Cell Death Dis. 7:e2461. PubMed
  40. Jozic I, et al. 2021. Commun Biol. 4:757. PubMed
  41. DiTommaso T, et al. 2014. PLoS Genet. 10:e1004706. PubMed
  42. Johnson J, et al. 2014. Cancer Genomics Proteomics. 11:115-126. PubMed
  43. Lin J, et al. 2016. Nature. 540:124-128. PubMed
  44. Shwartz Y, et al. 2020. Cell. 182:578. PubMed
  45. Norum JH, et al. 2019. Int J Cancer. web only. PubMed
  46. Kim CS, et al. 2020. Cell Metabolism. 32(4):629-642.e8. PubMed
  47. Anderton H, et al. 2018. Cell Death Differ. 26:877. PubMed
  48. Coelho P, et al. 2015. Open Bio. 5: 150209. PubMed
  49. Anderton H, et al. 2017. J Invest Dermatol. 137:2371. PubMed
  50. Seong J, et al. 2018. Development. 145:14. PubMed
  51. Cottle DL, et al. 2020. Cell Rep Med. 1:100129. PubMed
  52. Alrefai H, et al. 2016. Nat Commun. 7:11724. PubMed
RRID
AB_2734680 (BioLegend Cat. No. 905702)
AB_2565052 (BioLegend Cat. No. 905701)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
16687 View all products for this Gene ID
UniProt
View information about Keratin 6A on UniProt.org

Related FAQs

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Go To Top Version: 6    Revision Date: 02/04/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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