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Alexa Fluor® 488 anti-GFP Antibody

Pricing & Availability
Clone
FM264G (See other available formats)
Regulatory Status
RUO
Other Names
Green Fluorescent Protein
Isotype
Rat IgG2a, κ
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Product Citations
publications
FM264G_AF488_GFP_Antibody_FC_032514
GFP transfected CHO cells were fixed and permeabilized, and then intracellularly stained with anti-GFP (clone FM264G) Alexa Fluor® 488 (filled histogram) or isotype control rat IgG2a Alexa Fluor® 488 (open histogram).
  • FM264G_AF488_GFP_Antibody_FC_032514
    GFP transfected CHO cells were fixed and permeabilized, and then intracellularly stained with anti-GFP (clone FM264G) Alexa Fluor® 488 (filled histogram) or isotype control rat IgG2a Alexa Fluor® 488 (open histogram).
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
338007 25 tests $147
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338008 100 tests $311
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Description

Green fluorescent protein (GFP) was originally identified as a protein involved in bioluminescence, which is from the jellyfish Aequorea Victoria. It is widely used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces without the need for exogenous substrates or cofactors, or other intrinsic or extrinsic proteins, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Purified GFP is a 27 kD monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TLR9-GFP transfected cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry1.

Application References

(PubMed link indicates BioLegend citation)
  1. Stephen LA, et al. 2018. Dev. Cell. 47(1):122-132.e4. PubMed (ICC)
Product Citations
  1. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  2. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  3. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  4. Mastorakos P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8720 - 8725. PubMed
  5. Adriaenssens E, et al. 2020. Sci Rep. 10:8755. PubMed
  6. Schwartz DM et al. 2019. Immunity. 50(1):106-120 . PubMed
  7. Su W, et al. 2020. Cell Metabolism. 32(6):996-1011.e7. PubMed
  8. An J, et al. 2022. iScience. 25:103570. PubMed
  9. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  10. Peauroi EM, et al. 2022. iScience. 25:105510. PubMed
  11. Gabriel SS, et al. 2021. Immunity. 54(8):1698-1714.e5. PubMed
  12. Moon HG et al. 2018. Immunity. 49(2):275-287 . PubMed
  13. Pettinato AM, et al. 2021. Cell Reports. 35(5):109088. PubMed
  14. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  15. Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed
  16. Shi H, et al. 2020. Immunity. 51(6):1012-1027.e7.. PubMed
  17. Doss PMIA, et al. 2021. Cell Reports. 34(10):108833. PubMed
  18. Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
  19. Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
  20. Tran NL, et al. 2022. Elife. 11:. PubMed
  21. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  22. Chen R, et al. 2019. Mol Med Rep. 1.172916667. PubMed
RRID
AB_2563287 (BioLegend Cat. No. 338007)
AB_2563288 (BioLegend Cat. No. 338008)

Antigen Details

Biology Area
Cell Biology, Immunology
Antigen References

1. Ishikura H, et al. 2004. Anticancer Res. 24:719.
2. Rizzuto R, et al. 1996. Curr. Biol. 6:183.
3. Chalfie M, et al. 1994. Science 263:802.

Gene ID
NA
UniProt
View information about GFP on UniProt.org

Related FAQs

Can I use common compensation control for GFP, CFSE and FITC because they emit in the same channel?
It is not recommended even if they emit in the same channel because these are still different fluors with different brightness intensities. Individual compensation controls should be employed.

Other Formats

View All GFP Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 488 anti-GFP FM264G ICFC,ICC
Purified anti-GFP FM264G ICFC,ICC
PE anti-GFP FM264G ICFC
Alexa Fluor® 647 anti-GFP FM264G ICFC,ICC
APC anti-GFP FM264G ICFC
PerCP/Cyanine5.5 anti-GFP FM264G ICFC
PE/Cyanine7 anti-GFP FM264G ICFC
Go To Top Version: 4    Revision Date: 12/16/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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