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- Clone
- J175-7D4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- ProMBP1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes surface stained with CD16 FITC, then intracellular stained with proMBP-1, clone J175-7D4, Alexa Fluor® 647 -
Human peripheral blood lymphocytes surface stained with CCR3 PE, then intracellular stained with proMBP-1, clone J175-7D4, Alexa Fluor® 647
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 346802 | 100 µg | $265 | ||||
Pro-form of Major Basic Protein 1 (ProMBP1) is composed of a strongly acidic 90-residue propart and a highly basic mature portion of 117 residues. It is an extensively and very heterogeneously glycosylated proteoglycan. ProMBP1 is processed to 14 kD MBP1 by convertase during maturation and localized in cell granule core. The mature MBP1 is a cytotoxic protein. The mAb J175-7D4 binds ProMBP1 and intermediate ProMBP1, but not mature MBP1. It specifically stain basophils, does not stain eosinophils, granulocytes, and mast cells and can be used as a marker for human basophils.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant pro-form of human major basic protein 1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ICFC - Quality tested
IP, IHC-P - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections
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Application References
(PubMed link indicates BioLegend citation) -
- Plager D, et al. 2006. J. Allergy Clin. Immunol. 117:626
- Product Citations
-
- RRID
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AB_2099170 (BioLegend Cat. No. 346802)
Antigen Details
- Structure
- 36-90kD heterogeneously glycosylated proteoglycan
- Distribution
-
basophils
- Cell Type
- Basophils
- Biology Area
- Immunology, Innate Immunity
- Gene ID
- 4153 View all products for this Gene ID
- UniProt
- View information about ProMBP1 on UniProt.org
Related FAQs
Other Formats
View All ProMBP1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human Pro-Major Basic Protein 1 (ProMBP1) | J175-7D4 | ICFC,IP,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Pro-Major Basic Protein 1 (ProMBP1)
Human peripheral blood lymphocytes surface stained with CD16... 
Human peripheral blood lymphocytes surface stained with CCR3...
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