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PE/Cyanine7 anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_PECy7_011909.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 PE/Cyanine7
  • G8dot8_PECy7_011909.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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118215 25 µg $124
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118216 100 µg $323
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zhou B, et al. 2018. J Clin Invest. 7:e1438105. PubMed
  2. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  3. Buechler MB, et al. 2021. Nature. 593:575. PubMed
  4. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  5. Teisanu R, et al. 2010. Am J Respir Cell Mol Biol. 44:794. PubMed
  6. Mourao L, et al. 2019. Sci Rep. 0.991666667. PubMed
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  11. Scott CL et al. 2018. Immunity. 49(2):312-325 . PubMed
  12. Ohigashi I, et al. 2021. Cell Rep. 36:109657. PubMed
  13. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
  14. Jahan S, et al. 2022. J Biol Chem. :102386. PubMed
  15. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  16. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  17. Morales RA, et al. 2022. Mucosal Immunol. 15:940. PubMed
  18. Ohigashi I, et al. 2020. Cell Reports. 29(9):2901-2916.e6.. PubMed
  19. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  20. Cen B, et al. 2021. Oncogene. 40:5984. PubMed
  21. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  22. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  23. Sheahan BJ, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:119. PubMed
  24. Osborne JK, et al. 2021. Cell Reports. 36(3):109408. PubMed
  25. Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
  26. Kass E, et al. 2016. Nat Commun. 7:13241. PubMed
  27. Lee JH et al. 2017. Cell. 170(6):1149-1163 . PubMed
  28. Dar HH, et al. 2022. JCI Insight. 7:. PubMed
  29. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  30. Sun P, et al. 2021. J Mammary Gland Biol Neoplasia. 26:377. PubMed
  31. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  32. Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed
  33. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  34. Tu WL, et al. 2018. Am J Pathol. 188:2688. PubMed
  35. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  36. Yip HYK, et al. 2018. PLoS One. 3. PubMed
  37. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  38. McNeil M, et al. 2021. J Mammary Gland Biol Neoplasia. 26:357. PubMed
  39. Pietzner J, et al. 2012. Hum Mol Genet. 122:493. PubMed
  40. Dost AFM, et al. 2020. Cell Stem Cell. 27(4):663-678.e8. PubMed
  41. Legrand JMD, et al. 2019. Nat Commun. 10:2278. PubMed
  42. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
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  44. Galani IE, et al. 2022. STAR Protoc. 3:101151. PubMed
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  46. Gonzalez M, et al. 2014. Proc Natl Acad Sci U S A. 111:3098. PubMed
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  49. Louie SM, et al. 2022. Cell Rep. 39:110662. PubMed
  50. Sountoulidis A, et al. 2012. PLoS One. 7:e41460. PubMed
  51. Kearns N, et al. 2013. Stem Cell Res. 11:1003. PubMed
  52. Nigro G, et al. 2014. Cell Host Microbe. 15:792. PubMed
  53. Napoli M, et al. 2022. Nat Commun. 13:614. PubMed
  54. Han Y, et al. 2022. Cell Rep. 38:110240. PubMed
  55. Nagatake T, et al. 2014. PLoS One. 9:90638. PubMed
  56. Liu B, et al. 2013. Development. 140:780. PubMed
  57. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  58. Magaletta ME, et al. 2022. Nat Commun. 13:457. PubMed
  59. Wang J, et al. 2022. Nat Protoc. 17:1359. PubMed
  60. Choi J, et al. 2020. Cell Stem Cell. 27:366. PubMed
  61. Liang Z, et al. 2017. Autophagy. 14:505. PubMed
  62. Nagatake T, et al. 2021. Int Immunol. 33:171. PubMed
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RRID
AB_1236477 (BioLegend Cat. No. 118215)
AB_1236471 (BioLegend Cat. No. 118216)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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