Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Raised against granulocytes of mouse origin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested
IP, IHC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁹. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁹. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4²⁰.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity², in vivo depletion^3-5,9, immunoprecipitation¹, immunohistochemical staining⁶ (including paraffin-embedded sections^9,16,33-35, acetone-fixed frozen sections¹¹ and zinc-fixed sections¹⁵), and Western blotting⁷. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)²⁰.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)

Product Citations

1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
2. Ahmed AU, et al. 2017. J Immunol. 199(6):2128. PubMed
3. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
4. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
5. Liu M, et al. 2022. J Exp Med. 219:. PubMed
6. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
7. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
8. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
9. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
10. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
11. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
12. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
13. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
14. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
15. Scalzo S, et al. 2022. Int J Nanomedicine. 17:2865. PubMed
16. Dubeykovskaya Z, et al. 2016. Nat Commun. 7:10517. PubMed
17. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
18. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
19. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
20. Moon H et al. 2017. Cell stem cell. 21(5):665-678 . PubMed
21. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
22. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
23. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
24. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
25. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
26. Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
27. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
28. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
29. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
30. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
31. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
32. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
33. Mikami H, et al. 2020. Nat Commun. 11:1162. PubMed
34. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
35. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
36. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
37. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
38. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
39. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
40. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
41. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
42. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
43. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
44. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
45. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
46. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
47. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
48. Köchl R, et al. 2020. Elife. 9:00. PubMed
49. Getts D, et al. 2008. J Exp Med. 205:2319. PubMed
50. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
51. Yu VWC, et al. 2017. Cell. 168:944. PubMed
52. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
53. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
54. Progatzky F, et al. 2021. Nature. 599:125. PubMed
55. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
56. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
57. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
58. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
59. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
60. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
61. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
62. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
63. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
64. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
65. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
66. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
67. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
68. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
69. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
70. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
71. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
72. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
73. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
74. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
75. Zeis P, et al. 2020. Immunity. 53:775. PubMed
76. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
77. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
78. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
79. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
80. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
81. Shin JW, et al. 2020. Cell Death Dis. 0.667361111. PubMed
82. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
83. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
84. Roderick J, et al. 2014. Proc Natl Acad Sci U S A. 111:14436. PubMed
85. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
86. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
87. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
88. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
89. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
90. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
91. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
92. Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed
93. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
94. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
95. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
96. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
97. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
98. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
99. Teng Y, et al. 2021. Mol Ther. 29:2424. PubMed
100. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
101. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
102. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
103. Olariu V, et al. 2021. Cell Reports. 34(2):108622. PubMed
104. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
105. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed

RRID

AB_313368 (BioLegend Cat. No. 108403)
AB_313369 (BioLegend Cat. No. 108404)

Antigen Details

Structure

21-25 kD

Distribution

Granulocytes, monocytes

Cell Type

Granulocytes, Monocytes, Neutrophils

Biology Area

Immunology, Innate Immunity

Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID

17067 View all products for this Gene ID 546644 View all products for this Gene ID

UniProt

View information about Ly-6G Ly-6C on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?

We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

Certificate of Analysis