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PE anti-mouse/rat CD49e Antibody

Pricing & Availability
Clone
HMα5-1 (See other available formats)
Regulatory Status
RUO
Other Names
α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
HMa5-1_PE_090707
C57BL/6 mouse bone marrow cells stained with HMα5-1 PE
  • HMa5-1_PE_090707
    C57BL/6 mouse bone marrow cells stained with HMα5-1 PE
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Cat # Size Price Quantity Check Availability Save
103905 50 µg $129
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103906 200 µg $323
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Description

CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, pro-B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The HMα5-1 antibody has been shown to partially block CD49e mediated interactions. It has also been observed that plate-bound HMα5-1 antibody enhances IgE mediated degranulation and that in vivo injection of HMα5-1 antibody, in combination with anti-CD49d and anti-CD61 antibodies, blocks passive cutaneous anaphylaxis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Affinity-Purified mouse VLA-5 protein (α5β1, CD49e/CD29)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vivo inhibition of fibronectin receptor (VLA-5) functions and in vitro blocking of CD49e mediated interactions 1,2. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103909 & 103910).

Application References

(PubMed link indicates BioLegend citation)
  1. Yasuda M, et al. 1995. Int. Immunol. 7:251. (IP Block)
  2. Noto K, et al. 1995. Int. Immunol. 7:835. (Block)
  3. Lin JS, et al. 2010. J. Leukoc. Biol. 88:95. PubMed
Product Citations
  1. Filippi A, et al. 2020. Cell Transplant. 29:963689720946277. PubMed
  2. Wang C, et al. 2009. Biomaterials. 30:2259. PubMed
RRID
AB_2128165 (BioLegend Cat. No. 103905)
AB_2265017 (BioLegend Cat. No. 103906)

Antigen Details

Structure
Integrin family, associates with integrin β1, 135 kD
Distribution

Activated T cells, mast cells

Function
Adhesion, costimulation
Ligand/Receptor
Fibronectin
Cell Type
Mast cells, Mesenchymal Stem Cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1994 Blood Cells 20:25.
3. Hemler ME. 1990. Annu. Rev. Immunol. 8:365.

Gene ID
16402 View all products for this Gene ID 315346 View all products for this Gene ID
UniProt
View information about CD49e on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 01/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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