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Purified anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Regulatory Status
RUO
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM48-1_PURE_CD48_Antibody_1_FC_061716
C57BL/6 mouse splenocytes stained with purified HM48-1, followed by anti-Armenian hamster IgG FITC.
  • HM48-1_PURE_CD48_Antibody_1_FC_061716
    C57BL/6 mouse splenocytes stained with purified HM48-1, followed by anti-Armenian hamster IgG FITC.
  • HM48-1_PURE_CD48_Antibody_3_IHCF_022819
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% rat serum + FBS for one hour at room temperature. Then the section was stained with 5 µg/ml of purified CD48 (clone HM48-1) and DyLight 594 Goat anti-hamster (Armenian) IgG secondary antibody (red), and 5 µg/ml of B220 (clone RA3-6B2) Brilliant Violet 510™ (yellow) overnight at 4°C. The image was captured with a 10X objective.
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103402 500 µg $136
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Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemical staining of frozen sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  2. Gao B, et al. 2018. Aging Cell. 3. PubMed
  3. Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed
  4. Hsieh HY, et al. 2020. Cancer Sci. 111:2400. PubMed
  5. Verbiest T, et al. 2018. Leukemia. 32:1435. PubMed
  6. Blriot C, et al. 2021. Immunity. :. PubMed
  7. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  8. Lin Z, et al. 2021. Stem Cells. 39:240. PubMed
RRID
AB_313017 (BioLegend Cat. No. 103402)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/20/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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