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PE anti-mouse/rat CD29 Antibody

Pricing & Availability
Clone
HMβ1-1 (See other available formats)
Regulatory Status
RUO
Other Names
integrin β1, VLA-β chain, β1 integrin, GPIIa, ITGB1
Isotype
Armenian Hamster IgG
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Product Citations
publications
Hmβ1-1
Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
  • Hmβ1-1
    Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
  • HMβ1-1_mouse
    Lou rat bone marrow cells stained with HMβ1-1 biotin, then detected with Sav-PE
Compare all formats See PE spectral data
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102207 50 µg $129
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102208 200 µg $353
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Description

CD29 is a 130 kD protein, also known as integrin β1, VLA-β chain, or GPIIa. It is a member of the integrin family, expressed broadly on leukocytes, endothelial cells, smooth muscle, and epithelial cells. In association with CD49a-f, CD29 forms the VLA-1 through VLA-6 complexes, respectively. It plays an important role in cell-cell or cell-matrix interaction. The HMß1-1 antibody reacts with both mouse and rat CD29. It is able to block cell adhesion and inhibit T cell proliferation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Purified mouse VLA-4 (α4β1, CD49d/CD29)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry4 of acetone-fixed frozen sections, in vitro blocking of the adhesion of mouse tumor cell lines to extracellular matrix proteins and in vitro inhibition of T cell proliferative responses1, and in vivo inhibition of neutrophil migration2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102235 and 102236).

Application References

(PubMed link indicates BioLegend citation)
  1. Noto K, et al. 1995. Int. Immunol. 7:835.
  2. Ridger VC, et al. 2001. J. Immunol. 166:3484.
  3. Jia W, et al. 2005. Blood 106:3854.PubMed
  4. Economopoulou M, et al. 2005. Blood 106:3831.
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  6. Eisenmann KM, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M703243200.PubMed
  7. Hayashi Y, et al. 2008. Am J Physiol Gastrointest Liver Physiol. 294:G778. PubMed
  8. Kim DT, et al. 2008. Blood 111:2929.PubMed
  9. Hayashi Y, et al. 2008. J Pharmacol Exp Ther. 326:523. PubMed
  10. Carlson TR, et al. 2008. Development. 135:2193. PubMed
  11. Sangaletti S, et al. 2008. Cancer Res. 68:9050. (Block) PubMed
  12. Baker CM, et al. 2012. PNAS. PubMed.
  13. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
Product Citations
  1. Guo SS, et al. 2020. Development. . PubMed
  2. Wei H, et al. 2021. Mol Med Rep. 23:00. PubMed
  3. Vallant N, et al. 2021. Int J Mol Sci. 22:00. PubMed
  4. Bo Yu et al. 2018. Cell stem cell. 23(2):193-209 . PubMed
  5. Benito-Jardón M, et al. 2020. J Cell Biol. 219: . PubMed
  6. Li C, et al. 2022. Neural Regen Res. 17:2050. PubMed
  7. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
  8. Larouche JA, et al. 2021. eLife. 0.416666666666667. PubMed
  9. Fan C, et al. 2020. Stem Cell Res Ther. 0.765277778. PubMed
  10. Yin M, et al. 2019. Int J Mol Med. 43:2086. PubMed
  11. Lan WR, et al. 2019. Stem Cells Int. 2019:8404168. PubMed
  12. Lai G, et al. 2022. PeerJ. 10:e13744. PubMed
  13. Weckbach L, et al. 2014. Blood. 123:1887. PubMed
  14. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  15. Tang S, et al. 2021. Front Cell Dev Biol. 9:670913. PubMed
  16. Mittal S, et al. 2016. Stem Cell Reports. 7:583-590. PubMed
  17. Huang L, et al. 2016. J Biol Chem. 291: 6232 - 6244. PubMed
  18. Toda S, et al. 2014. Blood. 123:3963. PubMed
  19. Zuba-Surma E, et al. 2008. J Mol Cell Cardiol. 44:865. PubMed
  20. Gross ETE, et al. 2018. Oncoimmunology. 8:e1404212. PubMed
  21. Jiang M, et al. 2020. Exp Ther Med. 1.989583333. PubMed
  22. Chen NP, et al. 2022. Nat Cell Biol. 24:723. PubMed
  23. Paterson N, et al. 2022. Elife. 11:. PubMed
  24. Diaferia G, et al. 2013. Development. 140:3360. PubMed
  25. Moretti FA, et al. 2018. Elife. 7:e35816. PubMed
  26. Shcherbina A, et al. 2020. Cell Rep. 32:107964. PubMed
  27. Yamamoto M, et al. 2019. Commun Biol. 2:292. PubMed
  28. Zaghdoudi S, et al. 2020. EMBO Mol Med. 12:e12010. PubMed
  29. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  30. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  31. Simonetti G, et al. 2013. J Exp Med. 210:2887. PubMed
  32. Zografou A, et al. 2011. J Plast Reconstr Aesthet Surg. 64:1647. PubMed
  33. Zheng Z, et al. 2012. Biochem Biophys Res Commun. 428:309. PubMed
  34. Kurzrock E, et al. 2008. Am J Physiol Renal Physiol. 294:F1415. PubMed
  35. Masgutov R, et al. 2018. Front Pharmacol. 9:86. PubMed
  36. Stepp M, et al. 2007. J Cell Sci. 120:2851. PubMed
  37. Izawa Y, et al. 2018. J Cereb Blood Flow Metab. 38:641. PubMed
  38. Shi C, et al. 2015. Biomaterials. 58: 10-25. PubMed
  39. Deng P, et al. 2021. Cell Stem Cell. 28(6):1057-1073.e7. PubMed
  40. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
  41. Si H, et al. 2018. Int J Mol Med. 41:3342. PubMed
  42. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  43. Bauer M, et al. 2009. Proc Natl Acad Sci U S A. 106:1920. PubMed
  44. Liang C, et al. 2013. Acta Biomater. 9:9423. PubMed
  45. Cizkova D, et al. 2014. Sci Rep. 4:7514. PubMed
  46. Cobbold SP, et al. 2018. Front Immunol. 9:1381. PubMed
  47. Wang T, et al. 2022. Nat Commun. 13:160. PubMed
RRID
AB_312884 (BioLegend Cat. No. 102207)
AB_312885 (BioLegend Cat. No. 102208)

Antigen Details

Structure
Integrin family, 130 kD
Distribution

Leukocytes, endothelial cells, smooth muscle, epithelial cells

Function
Adhesion
Ligand/Receptor
Extracellular matrix
Cell Type
Embryonic Stem Cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Noto K, et al. 1995. Int. Immunol. 7:835.
2. Springer TA. 1990. Nature 346:425.

Gene ID
16412 View all products for this Gene ID 24511 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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