Loading...

SARS-CoV-2 Reactive T cell Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Lymphocyte function-associated antigen 1, LFA-1, antigen specific, T cells, CD11a/CD18
Ave. Rating
Submit a Review
Product Citations
publications
A_345903_SARSCoV2_Reactive_T_cell_kit
Kit content image for the SARS-CoV-2 Reactive T cell Kit
  • A_345903_SARSCoV2_Reactive_T_cell_kit
    Kit content image for the SARS-CoV-2 Reactive T cell Kit
  • B_SARS-CoV-2-Reactive_T-cell-Kit_042922
    CD8+ human peripheral blood lymphocytes were stimulated lyophilized negative control (left) or SARS-CoV-2 peptide pool (right) for 20 minutes, then stained with anti-human LFA-1 PE followed by surface cocktail. Data was analyzed using gating strategy depicted in Figure below.
  • C_SARS-CoV-2-Reactive_T-cell-Kit__042722
    CD4+ human peripheral blood lymphocytes were stimulated with lyophilized negative control (left) or SARS-CoV-2 peptide pool (right) for 4 hours, then stained with anti-human LFA-1 PE followed by surface cocktail. Data was analyzed using gating strategy depicted in Figure below.
  • D_SARS-CoV-2-Reactive_T-cell-Kit__042722
    Gating strategy using scatter parameters and surface markers in the staining cocktail to identify CD4+ and CD8+ cells.
Cat # Size Price Quantity Check Availability Save
345903 10 tests $464
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The SARS-CoV-2 reactive T cell kit contains SARS-CoV-2 peptide pool (tube S), negative control (tube N) and positive control (tube P) in a convenient packaging for stimulating human blood/ PBMCs along with anti-human LFA-1 PE and a surface stain cocktail consisting of anti-human CD45 PerCP, CD8 Pacific Blue™, CD4 FITC and CD14, CD41, CD15, CD16, CD19 APC/Cyanine7. The kit can be used for assessing both SARS-CoV-2 specific responses and vaccine responses. Cells or beads stained with individual fluorochrome-conjugated antibodies should be used for setting up compensation before running the samples. For data analysis, we recommend following gating strategy – Time –> Singlets –> Lymphocytes –> APC/Cyanine7 (Dump) negativeCD45+ –> CD4+CD8– and CD8+CD4–.

The SARS-CoV-2 reactive T cell kit provides a convenient, easy to use, and powerful tool for immunology and vaccine research.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Formulation
Each kit will contain the following components:

Kit Components:

1 vial negative control
1 vial positive control
1 vial SARS-CoV-2 Spike (Glycoprotein) peptide pool
1 vial surface stain cocktail (5 tests)
3 tubes of LFA-1 PE (1 test each)
1 bottle 50ml RBC Lysis/Fixation solution
1 bottle 0.5ml EDTA 0.08M
1 bottle 500ml Cell Staining Buffer
Preparation
Kit consists of lyophilized components.
Storage & Handling
Store the kit at 2°C - 8°C upon receipt. Do not open the sealed pouch until ready to use. One pouch contains sufficient reagents to test one donor sample.
Application

FC - Quality tested
Recommended Usage

Refer to manual for detailed usage instructions.

Application References

(PubMed link indicates BioLegend citation)
  1. Dimitrov S, et al. 2018. Pro Natl Acad Sci  U S A. 115:E5536-E5545.
  2. Gouttefageas C, et al. 2019. Cancer Immunol Immunother. 68:1855-1863.
  3. Schollhorn A, et al. 2021. Front Immunol. 12:626308.
  4. Schollhorn A, et al. 2023. Front Immunol. 13:1107366.

Antigen Details

Interaction
CD4+ T cells, CD8+ T cells, SARS-CoV-2, antigen specificity
Ligand/Receptor
β2-integrin
Antigen References
  1. Kundu R, et al. 2022. Nature Comm. 13:80.
  2. Lou XH, et al. 2021. Scand J Immunol. 93:e12989.
  3. Canete PF, et al. 2020. Cell. 183:13.
  4. Sette A, et al. 2021. Cell. 184:861.
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/27/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account