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- Clone
- 49.2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T Cell Receptor Vγ4, TCR Vγ2, TRGV4, GV3S1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 splenocytes were stained with purified anti-mouse TCR Vγ4 (clone 49.2) (left) or purified mouse IgG1, κ isotype control (right) followed by rat anti-mouse IgG1 PE. Cells were co-stained with anti-CD3 FITC and anti-γδ TCR APC. Data shown are CD3+ gated cells.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 160602 | 500 µg | $581 | ||||
T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex. The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation. γδ T cells (gamma delta T cells) are a small subset of T cells that express a unique TCR. They are found in the thymus, peripheral lymphoid tissue, and peritoneum. Unlike αβ T cells, γδ T cells do not require antigen processing in order to recognize certain bacterial, lipid, and tumor antigens.
The 49.2 antibody reacts specifically with mouse TCR Vγ4 as designated by Heilig/Tonegawa nomenclature.2 Mouse TCR Vγ4 is also known as TCR Vγ2 under the Garman nomenclature system. Several TCR γ and δ chain nomenclature systems exist.
In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- C57BL/6 thymocytes expanded in vitro in anti-TCRγδ antibody coated plates
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Khairallah C, et al. 2015. PLoS Pathog. 11:e1004702.
- Perira P, et al. 2000. Eur J Immunol. 30:1988-97.
- RRID
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AB_2876572 (BioLegend Cat. No. 160602)
Antigen Details
- Structure
- Heterodimer with γ and δ chain
- Distribution
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Subset of γδ T cells
- Function
- Antigen recognition
- Interaction
- CD3
- Cell Type
- Lymphocytes, T cells, Thymocytes
- Biology Area
- Adaptive Immunity, Immunology, Innate Immunity
- Molecular Family
- TCRs
- Gene ID
- 21638 View all products for this Gene ID
- UniProt
- View information about TCR Vgamma4 on UniProt.org
Related FAQs
Other Formats
View All TCR Vγ4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-mouse TCR Vγ4 | 49.2 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse TCR Vγ4
C57BL/6 splenocytes were stained with purified anti-mouse TC...
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