PE anti-mouse CD321 Antibody

Pricing & Availability
Clone
90G4 (See other available formats)
Regulatory Status
RUO
Other Names
Junctional Adhesion Molecule A (JAM-A), JAM-1, or F11 Receptor, Jcam, Jcam1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
90G4_PE_CD321_Antibody_070920.png
C57BL/6 whole blood was stained with CD45, CD41 and CD321 (clone 90G4) PE (left) or rat IgG2a, κ PE isotype control (right). Dot plots are gated on CD45- cells.
  • 90G4_PE_CD321_Antibody_070920.png
    C57BL/6 whole blood was stained with CD45, CD41 and CD321 (clone 90G4) PE (left) or rat IgG2a, κ PE isotype control (right). Dot plots are gated on CD45- cells.
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158503 25 µg $117
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158504 100 µg $292
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Description

CD321, also known as Junctional Adhesion Molecule A (JAM-A), JAM-1, or F11 Receptor, is a 32-40 kD type I transmembrane glycoprotein which belongs to the immunoglobin superfamily. It is expressed broadly on leukocytes, platelets, erythrocytes, and epithelial and endothelial cells. CD321 plays a role in cell adhesion, leukocyte migration, and angiogenesis, through its interactions with integrins, such as LFA-1 and αvβ3 integrins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse MS-1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2876548 (BioLegend Cat. No. 158503)
AB_2876548 (BioLegend Cat. No. 158504)

Antigen Details

Distribution

Leukocytes, platelets, erythrocytes, and epithelial and endothelial cells.

Cell Type
Endothelial cells, Epithelial cells, Leukocytes, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Fukuhara T, et al. 2017. PLoS One. 12(10)
Gene ID
16456 View all products for this Gene ID
UniProt
View information about CD321 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/09/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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