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PE anti-mouse TIGIT (Vstm3) Antibody

Pricing & Availability
Clone
A17200C (See other available formats)
Regulatory Status
RUO
Other Names
T-Cell Immunoreceptor with Ig and ITIM domains, T cell Ig, Immunoglobulin Domain-Containing Protein 9, V-Set, Transmembrane Domain-Containing Protein 3, WUCAM
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A17200C_PE_TIGIT_Antibody_013120.png
ConA-stimulated (3 days) C57BL/6 mouse splenocytes were stained with PE anti-mouse TIGIT (Vstm3) (clone A17200C) (left) or PE Rat IgG2a, κ isotype control (right) and APC CD3.
  • A17200C_PE_TIGIT_Antibody_013120.png
    ConA-stimulated (3 days) C57BL/6 mouse splenocytes were stained with PE anti-mouse TIGIT (Vstm3) (clone A17200C) (left) or PE Rat IgG2a, κ isotype control (right) and APC CD3.
See PE spectral data
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622205 25 µg $141
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622206 100 µg $347
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Description

TIGIT (T cell immunoreceptor with Ig and ITIM domains precursor) contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif. TIGIT is an inhibitory receptor expressed on the surface of natural killer (NK) cells. TIGIT recognizes nectin and nectin-like adhesion molecules and thus plays a critical role in the innate immune response to malignant transformation. TIGIT in conjunction with CD226 forms a pathway that has prominent similarities to the costimulatory CD28/CTLA-4 pathway. CD226 and TIGIT bind the same set of ligands (CD155 and CD112) and CD226 is a positive regulator of T cell responses, while TIGIT inhibits them. Knockdown of TIGIT expression in human CD4+ T cells results in an increase of both T-bet and IFN-γ mRNA and protein expression. These effects are overcome by blocking CD226 signaling indicating that TIGIT exerts immunosuppressive effects by competing with CD226 for the same ligand, CD155. The binding of CD155 to TIGIT on human dendritic cells enhances the production of interleukin 10 and diminishes the production of interleukin 12p40. TIGIT shows an inhibitory effect on cell-signaling pathway in murine models of experimental autoimmune encephalomyelitis (EAE). Loss of TIGIT expression in mice results in hyperproliferative T cell responses and increased susceptibility to EAE.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TIGIT Recombinant Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2832837 (BioLegend Cat. No. 622205)
AB_2832838 (BioLegend Cat. No. 622206)

Antigen Details

Structure
Homodimer
Distribution

Human follicular B helper T cells (TFH), regulatory, memory and activated T cells, NK cells.

Function
Immunosuppressive effects by binding CD155 and modulating cytokine production by DC. Inhibits NK cell cytotoxicity. Inhibits T cell functions by competing with CD226. Tregs TIGIT+ cells suppress proinflammatory Th1 and Th17 cells, but not Th2 cell responses.
Interaction
Dendritic cells, monocytes
Ligand/Receptor
Poliovirus receptor (PVR/CD155) and nectin-2 (PRR-2/CD112)
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Yu X, et al. 2009. Nat. Immunol. 10:48.
  2. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. U S A. 106:17858.
  3. Joller N, et al. 2011. J. Immunol. 186:1338.
  4. Tahara-Hanaoka S, et al. 2004. Int. Immunol. 16:533.
  5. Lozano E, et al. 2012. J. Immunol. 188:3869.
  6. Joller N, et al. 2014. Immunity. 40:569.
  7. Deuss FA, et al. 2017. J. Biol. Chem. 292:11413.
Gene ID
100043314 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/30/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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