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Purified anti-human Uncarboxylated Osteocalcin Antibody

Pricing & Availability
Clone
8H4 (See other available formats)
Regulatory Status
RUO
Other Names
Osteocalcin, uncarboxylated osteocalcin, decarboxylated osteocalcin, 3xGlu, bone gamma-carboxyglutamate protein, Bone Gla Protein, BGP, OC, OCN, unOCN
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
8H4_PURE_Uncarboxylated-Osteocalcin_Antibody_1_112719
Representative sandwich immunoassay standard curve generated using serial dilutions of Human Uncarboxylated Osteocalcin (Cat. No. 446709) ranging from 37.5 to 2400 pg/mL, 8H4 (Cat. No. 538202) as the capture antibody at 4 µg/mL, and 4B6 (Cat. No. 538304) as the detection antibody at 0.5 µg/mL.
  • 8H4_PURE_Uncarboxylated-Osteocalcin_Antibody_1_112719
    Representative sandwich immunoassay standard curve generated using serial dilutions of Human Uncarboxylated Osteocalcin (Cat. No. 446709) ranging from 37.5 to 2400 pg/mL, 8H4 (Cat. No. 538202) as the capture antibody at 4 µg/mL, and 4B6 (Cat. No. 538304) as the detection antibody at 0.5 µg/mL.
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538202 500 µg $581
 
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Description

Osteocalcin is the most abundant non-collagenous protein found in the bone. While fully-carboxylated osteocalcin has a high affinity for the extracellular matrix of the bone; decreases in pH, caused by the process of bone resorption, result in decarboxylation of this protein. This generates both partially decarboxylated (undercarboxylated) and fully decarboxylated (uncarboxylated) osteocalcin molecules which are released into the circulation due to decreased affinity for the extracellular matrix. Fully carboxylated osteocalcin requires high calcium concentrations for proper protein folding; however, under/uncarboxylated osteocalcin has no calcium requirement to maintain its structure within the blood.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human Decarboxylated (3xGLU) Osteocalcin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1.0 - 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of Human Uncarboxylated Osteocalcin recombinant protein (Cat. No. 446709) ranging from 37.5 to 2400 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The pure 8H4 antibody (Cat. No. 538202) is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 4B6 antibody (Cat. No. 538304) as the detection antibody and Human Uncarboxylated Osteocalcin synthetic peptide as the ELISA Std. (Cat. No. 446709).

RRID
AB_2888730 (BioLegend Cat. No. 538202)

Antigen Details

Structure
Monomer
Distribution

It is produced by osteoblasts as a 49 amino acid protein, which contains 3 glutamine acid residues (Glu 17, 21, and 24) that are gamma carboxylated in a vitamin K dependent manner.

Function
Under/uncarboxylated forms act as hormones promoting β-cell proliferation, adiponectin secretion, insulin sensitivity, glucose tolerance, and testosterone biosynthesis. In patients with diabetes, reduced serum levels of osteocalcin are negatively correlated with obesity and insulin resistance. Furthermore, supporting studies in mice have suggested potential therapeutic applications for osteocalcin in both obesity and insulin resistance.
Interaction
GPRC6A, insulin, pancreatic β-cells, Ley-dig cells, adiponectin
Ligand/Receptor
Under/uncarboxylated forms can bind the receptor GPCR6a, calcium, and metal-binding.
Cell Type
Osteoblasts
Molecular Family
Hormones
Antigen References
  1. Booth SL, et al. 2013. Nat Rev Endocrinol. 9:43.
  2. Diaz-Franco MC, et al. 2019. Mol Med Rep. 19:15-22.
  3. Karsenty G & Oury F. 2014. Mol Cell Endocrinol. 382:521.
  4. Zoch ML, et al. 2016. Bone. 82:42-9.
Gene ID
632 View all products for this Gene ID
UniProt
View information about Uncarboxylated Osteocalcin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/27/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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