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Ultra-LEAF™ Purified anti-human CD200 (OX2) Antibody

Pricing & Availability
Clone
OX-104 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70655
Other Names
OX-2, OX2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_OX-104_Purified_CD200_Antibody_031219_updated.png
Human peripheral blood lymphocytes were stained with CD4 APC and CD200 (OX2) (clone OX-104) Purified (left) or Mouse IgG1, κ isotype control (right) followed by Biotin anti-Mouse IgG and Streptavidin-PE.
  • 1_OX-104_Purified_CD200_Antibody_031219_updated.png
    Human peripheral blood lymphocytes were stained with CD4 APC and CD200 (OX2) (clone OX-104) Purified (left) or Mouse IgG1, κ isotype control (right) followed by Biotin anti-Mouse IgG and Streptavidin-PE.
  • 2_OX-104_ULEAF_CD200_Antibody_1_052819.png
    Recombinant biotinylated hCD200 (Black circles, Cat. No. 770206) binds to immobilized recombinant hCD200R (Cat. No. 770106). Anti-hCD200 antibody (Clone OX-104, Purple squares) inhibits the binding in a dose-dependent manner. This antibody blocks binding of 40 ng/mL of hCD200 biotinylated protein to 0.5 µg/mL immobilized recombinant hCD200R with an ND50 = 0.4 - 2 µg/mL.
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329226 100 µg $248
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329227 1 mg $715
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Description

CD200, also known as OX2, is a member of the immunoglobulin superfamily (IgSF). It is a monomorphic cell surface glycoprotein that is expressed on thymocytes, neurons, endothelium, follicular dendritic cells in all lymphoid organs, a subset of CD34+ progenitor cells, and at low levels on some smooth muscle and B lymphocytes. It is not expressed on NK cells, monocytes, granulocytes, or platelets. CD200 costimulates T cell proliferation. It may regulate myeloid cell activity in a variety of tissues. The interaction between CD200 (OX2) and CD200 receptor (OX2R) system is of importance in the control of macrophage and granulocyte activation, which may contribute to pathways that suppress and limit macrophage induced inflammatory damage in tissue.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Verified
IHC-F, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For blocking application, the ND50 range: 0.4 - 2.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of formalin-fixed paraffin-embedded sections1 and acetone-fixed frozen sections2, and blocking of CD200 interaction with CD200R.

Application References

(PubMed link indicates BioLegend citation)
  1. Patel GK, et al. 2012. J. Invest. Dermatol. 132:401. (IHC)
  2. Wright GJ, et al. 2001. Immunology 102:173. (IHC)
  3. Foster-Cuevas M, et al. 2004. J. Virol. 78:7667. (FC)
RRID
AB_2810502 (BioLegend Cat. No. 329226)
AB_2810503 (BioLegend Cat. No. 329227)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

T cells, neurons, endothelium

Function
Costimulates T cell proliferation
Receptors
CD200R
Cell Type
Endothelial cells, Neurons, T cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Wright GJ, et al. 2001. Immunol. 102:173.
2. Foster-Cuevas M, et al. 2004. J. Virol. 78:7667.
3. Mason D, et al. 2002. ed. Leukocyte Typing VII. New York:Oxford Univ. Press.
4. Broderick C, et al. 2002. Am. J. Pathol. 161:1669.

Regulation
Induces a downregulation of macrophage activity
Gene ID
4345 View all products for this Gene ID
UniProt
View information about CD200 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 05/28/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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