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APC anti-human CD300F (IREM-1) Antibody

Pricing & Availability
Clone
UP-D2 (See other available formats)
Regulatory Status
RUO
Other Names
CD300LF, IREM-1, IgSF13
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
UP-D2_APC_CD300F_Antibody_031218
Human peripheral blood monocytes were stained with CD300F (clone UP-D2) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • UP-D2_APC_CD300F_Antibody_031218
    Human peripheral blood monocytes were stained with CD300F (clone UP-D2) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
See APC spectral data
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340605 25 tests $131
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340606 100 tests $362
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Description

IREM-1 (immune receptor expressed by myeloid cell 1) is a type I single transmembrane protein encoded by human chromosome 17q24.25. A member of the CD300 (CMRF-35) family and the Ig superfamily, IREM-1 is also known as CD300F, or CD300LF (CD300-like member F). CD300F is expressed on monocytes, granulocytes, and myeloid leukemia cell lines. The cytoplasmic domain of IREM-1 contains an ITIM and associates with the P85α subunit of PI3K (phosphoinositide 3-kinase). IREM-1 also functions as an inhibitory receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: Coengagement of IREM-1 with UP-D2 inhibits FcReI-mediated degranulation, immunoprecipitation

Application References

(PubMed link indicates BioLegend citation)
  1. Alvarez-Errico D, et al. 2004. Eur. J. Immunol. 34:3690
  2. Lee SM, et al. 2011. J. Immunol. 186:6296. PubMed.
RRID
AB_2728305 (BioLegend Cat. No. 340605)
AB_2728306 (BioLegend Cat. No. 340606)

Antigen Details

Structure
Type I single transmembrane protein with cytoplamic ITIM, associates with p85α of PI3K, Ig superfamily
Distribution

Monocytes, granulocytes, and myeloid leukemia cell lines

Function
Inhibition of myeloid function
Cell Type
Monocytes, Granulocytes, Leukemia
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Marquez JA, et al. 2007. J. Mol. Bio. 367:310
2. Alvarez-Errico D, et al. 2004. Eur. J. Immunol. 34:3690
3. Alvarez-Errico D, et al. 2007. J. Immunol. 178:808

Gene ID
146722 View all products for this Gene ID
UniProt
View information about CD300F on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/13/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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