- Clone
- IIB1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
668505 | 100 µg | $382 |
TFIIB is one of the ubiquitous transcription factors required for transcription initiation by RNA polymerase II. It plays an important role in pre-initiation complex assembly, which provides a bridge between promoter-bound TFIID and RNA polymerase II. Moreover, TFIIB also directly modulates the catalytic center of RNA polymerase II.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
Clone IIB1 recognizes epitope 1-51.
-
Application References
(PubMed link indicates BioLegend citation) -
- Thompson NE, et al. 1995. J. Biol. Chem. 270:4735. (IP)
- RRID
-
AB_2566350 (BioLegend Cat. No. 668505)
Antigen Details
- Structure
- 316 amino acids with a predicted molecular weight of approximately 35 kD.
- Distribution
-
Nucleus.
- Function
- The Transcription factor is required for transcription initiation by RNA polymerase II.
- Interaction
- Transcription factors IID and IIA and RNA polymerase II.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Ha I, et al. 1991. Nature 352:689.
3. Hurwitz, J. 2005. J. Biol. Chem. 280:42477.
4. Deng W, Roberts SG. 2007. Chromosoma. 5:417.
5. Hirata A, et al. 2008. Nature 451:851. - Gene ID
- 2959 View all products for this Gene ID
- UniProt
- View information about TFIIB on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All TFIIB Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TFIIB | IIB1 | WB,ICC,IP |
Alexa Fluor® 594 anti-TFIIB | IIB1 | ICC |
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