Loading...

Purified anti-Estrogen Receptor Antibody (Previously Covance catalog# SIG-3665)

Pricing & Availability
Clone
D75 (See other available formats)
Regulatory Status
RUO
Other Names
ESR, ESRA, ESTRR, Era, NR3A, Estrogen receptor, ER-alpha, estradiol receptor, estrogen receptor alpha delta 4 +49 isoform, nuclear receptor subfamily 3 group A member 1
Previously
Signet Catalog# 665-01
Covance Catalog# SIG-3665
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
D75_PURE_estrogen_Antibody_122018_updated.png
IHC staining of purified anti-Estrogen Receptor antibody (clone D75) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with a 1:40 dilution of the primary antibody for 60 minutes at room temperature. Following a biotinylated goat anti-rat linking reagent, BioLegend's Ultra-Streptavidin (USA) HRP labeling reagent and DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D75_PURE_estrogen_Antibody_122018_updated.png
    IHC staining of purified anti-Estrogen Receptor antibody (clone D75) on formalin-fixed paraffin-embedded human breast tumor tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with a 1:40 dilution of the primary antibody for 60 minutes at room temperature. Following a biotinylated goat anti-rat linking reagent, BioLegend's Ultra-Streptavidin (USA) HRP labeling reagent and DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D75_PURE_estrogen_Antibody_2_012422
    IHC staining of anti-Estrogen Receptor antibody (clone D75) on formalin-fixed paraffin-embedded Human Breast Tumor tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1, the tissue was incubated with the primary antibody at 1:40 dilution for 60 min at room temperature. Biolegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
Cat # Size Price Quantity Check Availability Save
916201 1 mL $496
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, the suggested dilution is 1:40. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone D75 recognizes the human estrogen receptor a.

Application References

(PubMed link indicates BioLegend citation)
  1. Schultz J, et al. 2002. J Biol Chem. 277:8702.
  2. Zivadinovic D, Watson CS. 2005. Breast Cancer Res. 7:R130.
Product Citations
  1. Nguyen QH, et al. 2018. Nat Commun. 9:902. PubMed
RRID
AB_2565176 (BioLegend Cat. No. 916201)

Antigen Details

Cell Type
Mesenchymal Stem Cells
Biology Area
Stem Cells
Gene ID
2099 View all products for this Gene ID
UniProt
View information about Estrogen Receptor on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 02/08/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account