Purified anti-IRF6 Antibody anti-IRF6

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Clone: 14B2C16 (See other available formats)
Regulatory Status: RUO
Other Names: Interferon regulatory factor 6, IRF-6
Isotype: Mouse IgG2a, κ
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Product Citations: publications

14B2C16_PURE_IRF6_Antibody_1_WB_071816 — Total lysates (15 µg protein) from Jurkat (lane 1), HaCaT (lane 2) and NIH3T3 (lane 3) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (0.5 µg/mL) purified anti-IRF6 antibody (upper). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (Cat. No. 405306) or HRP Donkey anti-rabbit IgG Antibody (Cat. No. 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, Cat. No. 631401) antibody was used as a loading control (lower). Lane M: MW ladder.

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674502

100 µg

$265

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Description

IRF6 is a member of the IRF family of transcription factors, which are based on the presence of the conserved IRF tryptophan pentad repeat DNA-binding domain. Unlike other IRF transcription factors, IRF6 is not involved in the expression of interferon genes and doesn’t seem to be involved in innate immunity. Data suggests that IRF6 is necessary for appropriate epidermal differentiation and development. Mutations of IRF6 are associated with human craniofacial developmental defects such as cleft lip, palate, tooth agenesis, Van der Woude syndrome, and popliteal pterygium syndrome. IRF6-deficient mice display severe morphological defects in skin and limbs, and result in perinatal lethality as well.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human IRF6 recombinant protein (92-223 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
ICFC, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2565216 (BioLegend Cat. No. 674502)

Antigen Details

Structure: 467 amino acids with a predicted molecular weight of 53 kD and contains an IRF trytophan pentad repeat that is responsible for DNA binding.
Distribution: Nucleus and cytoplasm.
Function: IRF6 is a transcriptional activator that regulates proliferation and differentiation of both epithelial cells and keratinocytes. IRF6 is required for normal epidermal development during embryogenesis.
Interaction: Interacts with SERPINB5.
Biology Area: Cell Biology, Transcription Factors
Antigen References: 1. Melath A, et al. 2013. Asian Pac. J. Cancer Prev. 14:6803.
2. Salahshourifar I, et al. 2012. Eur. J. Med. Genet. 55:389.
3. Blackburn J, et al. 2012. Dev. Biol. 365:61.
4. Botti E, et al. 2011. Proc. Natl. Acad. Sci. USA 108:13710.
5. Restivo G, et al. 2011. EMBO 30:4571.
6. Fleming JA, et al. 2009. Mol. Cell Endocrinol. 299:252.
Gene ID: 3664 View all products for this Gene ID
UniProt: View information about IRF6 on UniProt.org

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Other Formats

View All IRF6 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-IRF6	14B2C16	WB,ICFC,ICC
PE anti-IRF6	14B2C16	ICFC
APC anti-IRF6	14B2C16	ICFC

Certificate of Analysis

Go To Top Version: 6 Revision Date: 11/28/2017

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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