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PE anti-mouse/rat CD42d Antibody

Pricing & Availability
Clone
1C2 (See other available formats)
Regulatory Status
RUO
Other Names
Platelet glycoprotein V, GPV, platelet gpV, CD42d antigen, CD42d
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
1C2_PE_CD42d_Antibody_FC_091914
C57BL/6 platelets (resting) were stained with CD42d (clone 1C2) PE (filled histogram) or Armenian Hamster IgG PE istoype control (open histogram).
  • 1C2_PE_CD42d_Antibody_FC_091914
    C57BL/6 platelets (resting) were stained with CD42d (clone 1C2) PE (filled histogram) or Armenian Hamster IgG PE istoype control (open histogram).
Compare all formats See PE spectral data
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148503 25 µg $117
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148504 100 µg $214
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Description

CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX to form a receptor complex for von Willebrand factor on megakaryocytes and resting platelets. Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain of the GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex to release a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in Bernard-Soulier syndrome, a rare bleeding disorder.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse platelets
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Takada K, et al. 1995. Hybridoma. 14:361. (IP)
  2. Saito M, et al. 1996. Stem Cells. 14:124. (FC)
  3. Sato N, et al. 2000. Hybridoma. 19:455. (IP)
Product Citations
  1. Crescente M, et al. 2020. FASEB J. 34:10027. PubMed
RRID
AB_2564292 (BioLegend Cat. No. 148503)
AB_2564293 (BioLegend Cat. No. 148504)

Antigen Details

Structure
83 kD heterodimer composed of 2 polypeptide chains, an α chain (GP1BA) and a β chain (GP1BB).
Distribution
Resting platelets and megakaryocytes.
Function
CD42d is a component of the GPIb-V-IX complex, functions as a vWF receptor, and mediates vWF-dependent platelet adhesion to blood vessels.
Ligand/Receptor
CD42d.
Cell Type
Platelets, Megakaryocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sivaraman B, et al. 2011. Biomaterials. 32:5365.
2. Berger G, et al. 1996. Blood. 87:1385.
3. Ravanat C, et al. 1997. Blood. 89:3253.
4. Andrews RK, et al. 1998. Biochemistry. 37:638.
5. Inoue O, et al. 2008. 283:16279.
6. Hickey MJ, et al. 1993. Proc. Natl. Acad. Sci. 90:8327.
7. Shimomura T, et al. 1990. Blood. 75:2349.
8. Wu G, et al. 1997. Blood. 90:2660.

Gene ID
14729 View all products for this Gene ID
UniProt
View information about CD42d on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09/24/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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