Antibodies
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TruStain fcX™ (anti-mouse CD16/32) Antibody
TruStain fcX™ (anti-mouse CD16/32) Antibody
101319 50 µg $45.00     
101320 500 µg $195.00     
Product Details
 
Clone: 93 (See other available formats)
Isotype: Rat IgG2a, λ
Reactivity: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The CD16/32 antibody solution should be stored undiluted at 4°C.
Application:

FC - Quality tested
Recommended Usage:

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain fcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.
COA:
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Application
References:

  Publication Library



Description:

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain fcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.
Other Names: Fcγ R III/II, Ly-17
Structure: Ig superfamily, 40-60 kD
Distribution: B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
Function: Low affinity receptors for IgG
Ligand Receptor: IgG
GeneID: 14130
14131
Latest Publications: View the latest CD16/32 articles on HighwirePress.com
UniProt: View information about CD16/32 on UniProt.org
Keywords: TruStain fcX™ (anti-mouse CD16/32), 93 , Purified, Fcγ R III/II, Ly-17, Mouse, Flow Cytometry, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Cell Staining BufferFC, ICC, ICFC


Other Formats
DescriptionCloneApplications
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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