Each lot of this CD16/32 antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain fcX™ (anti-mouse CD16/32 antibody) (≤ 1.0 µg per 106 cells in 100 µl volume) for 5-10 minutes on ice prior to immunostaining.
Application References:
1. Oliver, A. M., et al., 1999. Hybridoma 18:113. 2. Brummel, R. and Lenert, P., 2005. J. Immunol. 174:2429.PubMed 4. Clements, J. L., et al., 2006. J. Immunol. 177:905.J Immunol 5. Flores, M.,et al.2008.FASEB J.PubMed 6. Ge, XN., et al. 2010. J. Immunol. 185:1205. PubMed.
Description:
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain fcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.
Other Names:
Fcγ R III/II, Ly-17
Structure:
Ig superfamily, 40-60 kD
Distribution:
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
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