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For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain fcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.
COA:
Application References:
1. Oliver AM, et al. 1999. Hybridoma 18:113. 2. Brummel R and Lenert P. 2005. J. Immunol. 174:2429. PubMed 3. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. 4. Clements JL, et al. 2006. J. Immunol. 177:905. PubMed 5. Flores M, et al. 2008. FASEB J. 22:3661. PubMed 6. Ge XN, et al. 2010. J. Immunol. 185:1205. PubMed 7. Maseda D, et al. 2012. J. Immunol. 188:1036. PubMed.
Description:
CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain fcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.
Other Names:
Fcγ R III/II, Ly-17
Structure:
Ig superfamily, 40-60 kD
Distribution:
B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
