At BioLegend, our Technical Services team is dedicated and trained to answer your questions on our products and their related applications. All of our technical service staff are highly qualified and experienced to address your needs. We welcome any suggestions or comments to help us achieve the highest level of service. For technical support, please call us at 1-877-246-5343 (toll free), or fax your inquiry to 858-455-9587, or send your email to: techserv@biolegend.com. Our Technical Services Department hours are 7:00 AM to 5:30 PM, Pacific Standard Time, Monday through Friday. For customers located outside USA, you may contact us directly by phone at 858-455-9588, by fax at 858-455-9587, or by email. We ask that you provide us with as much information as possible so we can assist you more efficiently.

• Biofunctional Antibodies (Interactive Word file)
   
• Antibody Cross Reactivity Reference Chart
   
• Fluorochromes for Flow Cytometry and Microscopy
   
• Mouse Alloantigens
   
• TNF Receptor and Ligand Superfamilies: Nomenclature and Interaction
   
• Chemokines and Their Receptors
   
• Fluorochrome Brightness Index Chart
   
• Guide to Choosing and Using Fluorochromes in Multicolor Flow Cytometry
   
• Expression of Common Surface Molecules on Blood Cells
   
• Interactive Human CD Molecule Chart (Excel file)
   
• Interactive Mouse CD Molecule Chart (Excel file)
   
• External Resources - Useful Links
   

• FOXP3 Intracellular Staining Protocol
   
• Cell Surface Immunofluorescence Staining Protocol
   
• Intracellular Cytokine Staining Protocol
   
• Stimulation Guide for Intracellular Staining of Cytokines/Chemokines
   
• Cytokine Neutralization with Antibodies (Interactive Excel file)
   
• Red Blood Cell Lysis Protocol
   
• T Cell Activation with anti-CD3 Antibodies Protocol
   
• Western Blotting Protocol
   
• Immunofluorescence Microscopy Protocol
   
• Immunoprecipitation Protocol
   
• Sandwich ELISA Protocol
   
• ELISPOT Protocol
   
• Immunohistochemistry Protocol for Frozen Sections
   
• Immunohistochemistry Protocol for Paraffin-Embedded Sections
   

• Proteins, Antibodies, and Conjugates
   
• LEAF™ Purified Antibodies
   
• ELISA MAX™ Standard and Deluxe Sets
   
• Cat. #320118 -- Alexa Fluor® 488 anti-human FOXP3 Flow Kit
   
• Cat. #420201 -- Cell Staining Buffer
   
• Cat. #420301 -- Red Blood Cell Lysis buffer (10x)
   
• Cat. #420401, 420402 -- 7-AAD
   
• Cat. #420601 -- Brefeldin A Solution (1000x)
   
• Cat. #420701 -- Monensin Solution (1000x)
   
• Cat. #420801 -- Fixation Buffer
   
• Cat. #421001 -- Permeabilization Wash Buffer (10x)
   
• Cat. #421101 -- TMB Substrate Reagent Set , Reagent A & B
   
• Cat. #421201 -- Assay Diluent (5x)
   
• Cat. #421301 -- Propidium Iodide Solution
   
• Cat. #421403 -- FoxP3 Fix/Perm Buffer Set
   

• What is the shelf life of BioLegend products?
   
• How should BioLegend reagents be stored?
   
• How are BioLegend reagents shipped?
   
• In what formats and for what applications do you offer your antibodies?
   
• Does BioLegend offer Custom Services?
   
• What is the advantage of ordering per test instead of per mg?
   
• What is BioLegend's quality policy?
   
• Why should I order from a distributor?
   
• When more than one clone is available for a particular specificity, how do I choose the right one?
   
• What are the concentrations of your antibodies?
   
• I am going to do a functional assay. Which grade of purified antibody should I choose (What is the LEAF™ purified format)?
   
• How do I choose the right isotype control for the primary antibody I'm using?
   
• How do I obtain a Material Safety Data Sheet (MSDS) for your products?
   
• How can I obtain a Certificate of Analysis for your products?
   
• When can I expect to receive my order if I order directly from BioLegend?
   
• I’m looking for reagents that are cross-reactive with a different species (e.g. Rhesus Monkey or Cynomolgus). How do I search for cross-reactive reagents?
   
• What are good fluorochrome dyes to use in multi-color staining for flow cytometry?
   
• What's the difference between PE/Cy5 and PE/Cy5.5 conjugates?
   
• What components are included in the ELISA MAX™ Sets?
   
• Do the ELISA MAX™ Deluxe Sets come with pre-coated plates?
   
• Can I mix reagents from different ELISA MAX™ Sets, or use them for other applications?
   
• How many cells should I add to each well? What happens if I add the cells before adding the antigen or mitogen?
   
• How should I coat the plates with antibody? How should the plates be stored after coating?
   
• How should I wash the ELISPOT plates?
   
• What are possible causes of High Background in my Western Blot?
   
• What are possible causes of No Signal/Poor Signal in my Western Blot?