Each lot of this Streptavidin-PerCP/Cy5.5 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Application Notes:
Streptavidin-PerCP/Cy5.5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
C57BL/6 splenocytes stained with biotinylated 145-2C11, followed by Sav-PerCP/Cy5.5
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-PerCP/Cy5.5 is useful for detecting biotinylated antibodies. The excitation of PerCP/Cy5.5 by 488 nm laser light induces a light emission maximum of 690 nm.
Other Names:
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
