Streptavidin is conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated Streptavidin.
Concentration:
0.2 mg/ml
Storage & Handling:
The Streptavidin-PE/Cy7 solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC, ICFC
Recommended Usage:
Each lot of this Streptavidin-PE/Cy7 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin - Phycoerythrin/Cy7 (PE/Cy7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application References:
1. Kamimura, D., et al., 2006. J. Immunol. 177: 306. 2. Chappaz, S., et al., 2007. Blood doi:10.1182/blood-2007-02-074245. 3. Anderson, S. M., et al., 2007. J. Exp. Med. doi:10.1084/jem.20062571.PubMed
Description:
Streptavidin binds to biotin with high affinity. Streptavidin - Phycoerythrin/Cy7 (PE/Cy7) is useful for detecting biotinylated antibodies. The excitation of PE/Cy7 by 488 nm laser light induces a light emission maximum of 775 nm.
