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Streptavidin-PE/Cy7
Material Safety Data Sheet (MSDS) Streptavidin-PE/Cy7     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Streptavidin-PE/Cy7
405206 100 µg $175.00     
Clone: Streptavidin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated Streptavidin.
Concentration: 0.2 mg/ml
Storage & Handling: The Streptavidin-PE/Cy7 solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC

Recommended Usage:

Each lot of this Streptavidin-PE/Cy7 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application
Notes:

Streptavidin - Phycoerythrin/Cy7 (PE/Cy7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.

Application
References:

1. Kamimura, D., et al., 2006. J. Immunol. 177: 306.
2. Chappaz, S., et al., 2007. Blood doi:10.1182/blood-2007-02-074245.
3. Anderson, S. M., et al., 2007. J. Exp. Med. doi:10.1084/jem.20062571.PubMed
4. Chappaz, S., et al. 2010. J. Immunol. 184:3562. PubMed.



Description:

Streptavidin binds to biotin with high affinity. Streptavidin - Phycoerythrin/Cy7 (PE/Cy7) is useful for detecting biotinylated antibodies. The excitation of PE/Cy7 by 488 nm laser light induces a light emission maximum of 775 nm.

Other Names: Streptavidin-Phycoerythrin/Cy7, SAv-PE/Cy7
Cell Staining BufferFC, ICC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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