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Streptavidin-PE/Cy5
Material Safety Data Sheet (MSDS) Streptavidin-PE/Cy5     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Streptavidin-PE/Cy5
405205 100 µg $100.00     
Clone: Streptavidin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated Streptavidin.
Concentration: 0.2 mg/ml
Storage & Handling: The Streptavidin-PE/Cy5 solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application: FC, ICFC
Recommended Usage: Each lot of this Streptavidin-PE/Cy5 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Streptavidin-Phycoerythrin/Cy5 (PE/Cy5) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application
References:
1. Wong, KA., et al. 2008. J. Immunol. 15:180.PubMed
2. Qi, Q., et al. 2009. Blood. PubMed
3. Imada, M., et al. 2009. Int immunol. PubMed
C57BL/6 splenocytes stained with 145-2C11

C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-PE/Cy5


Description: Streptavidin binds to biotin with high affinity. Streptavidin-PE/Cy5 is useful for detecting biotinylated antibodies. The excitation of PE/Cy5 by 488 nm laser light induces a light emission maximum of 670 nm.
Other Names: Streptavidin-Phycoerythrin/Cy5, SAv-PE/Cy5
Cell Staining Buffer (FBS)FC,ICC,ICFC