Each lot of this Streptavidin-PE/Cy5 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin-Phycoerythrin/Cy5 (PE/Cy5) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application References:
1. Wong, KA., et al. 2008. J. Immunol. 15:180.PubMed 2. Qi, Q., et al. 2009. Blood. PubMed 3. Imada, M., et al. 2009. Int immunol. PubMed 4. Song, R., et al. 2010. J. Virol. PubMed.
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-PE/Cy5
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-PE/Cy5 is useful for detecting biotinylated antibodies. The excitation of PE/Cy5 by 488 nm laser light induces a light emission maximum of 670 nm.
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