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Clone:
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Streptavidin
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Formulation:
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Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
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Preparation:
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Streptavidin is conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated Streptavidin. |
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Concentration:
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0.2 mg/ml |
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Storage & Handling:
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The Streptavidin-PE/Cy5 solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
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Application:
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FC, ICFC |
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Recommended Usage:
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Each lot of this Streptavidin-PE/Cy5 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application. |
Application
Notes:
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Streptavidin-Phycoerythrin/Cy5 (PE/Cy5) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. |
Application
References:
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1. Wong, KA., et al. 2008. J. Immunol. 15:180.PubMed
2. Qi, Q., et al. 2009. Blood. PubMed
3. Imada, M., et al. 2009. Int immunol. PubMed |
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C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-PE/Cy5
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