Each lot of this Streptavidin-PE is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin-Phycoerythrin (PE) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application References:
1. Chappaz, S., et al., 2007. Blood doi:10.1182/blood-2007-02-074245. 2. Nishimoto, KP.,et al. 2008. J Immunol. 181:4010.PubMed 3. Niki, T., et al. 2009. J. Biol. Chem. 284:32344. PubMed.
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm.
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