Streptavidin is conjugated with HRPC under optimal conditions. The solution is free of unconjugated HRP and unconjugated Streptavidin.
Storage & Handling:
The streptavidin-HRP solution should be stored undiluted at 4°C. Avoid exposure to sodium azide.
Application:
ELISA - Quality Tested ELISPOT*, WB*, IHC * - *This application has been reported in the literature.
Recommended Usage:
Each lot of this Streptavidin-HRP is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:1000 - 1:3000 to determine optimal conditions. For ELISPOT analysis, the reagent should be titrated between 1:500 - 1:1500 to determine optimal conditions. Avoid using biotin-containing solutions as diluents and solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin-HRP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Sav-HRP is recommended for ELISA, ELISPOT and Western blotting, when used with the relevant substrate system.
Application References:
1. Li, K-J., et al., 2006. J. Leukoc. Biol. doi: 10.1189/jlb.1105668.PubMed 2. Weldon, S., et al. 2009. J. Immunol. 183:8148. PubMed.
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-HRP is useful for detecting biotinylated antibodies, e.g., in ELISA, ELISPOT, IHC or Western blotting. For ELISA applications, HRP will act on soluble substrates, such as ABTS or TMB, to yield a colorimetric reaction. For ELISPOT or Western blotting applications, HRP will act on precipitating substrates, such as 4CN, to yield a colorimetric reaction.
