Each lot of this Streptavidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies.
Application References:
1. Schmutz, S., et al. 2009. J. Immunol. 183:2217. PubMed
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.
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