Each lot of this Streptavidin-Cy5 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin-Cy5 is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application References:
1. Hsu, H-C., et al., 2007. J. Immunol. 178:5357.
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-Cy5 is useful for detecting biotinylated antibodies. The excitation of Cy5 by 625-650 nm laser light induces a fluorescence emission maximum of 670 nm.
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