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Streptavidin-APC/Cy7
Material Safety Data Sheet (MSDS) Streptavidin-APC/Cy7     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Streptavidin-APC/Cy7
405208 100 µg $175.00     
Clone: Streptavidin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated Streptavidin.
Concentration: 0.2 mg/ml
Storage & Handling: The Streptavidin APC/Cy7 solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application: FC, ICFC
Recommended Usage: Each lot of this Streptavidin APC/Cy7 is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Streptavidin-Allophycocyanin/Cy7 (APC/Cy7) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application
References:
1. Maresz, K., et al., 2005. J. Neurochemistry 95:437.
2. Ponomarev, E. D., et al., 2006. J. Immunol. 176:1402.J Immunol
3. Ponomarev, E. D., et al., 2007. J. Immunol. 178:39.
4. Mann, M. K., et al., 2007. J. Immunol. 178:3447.PubMed
5. Ponomarev, E.D.,et al.2005.J. Immunol Methods.300:32. PubMed
6. Cao, YA., et al. 2008. Blood.PubMed
7. Garidou, L., et al. 2009. J. Virol. PubMed
8. Dolgachev, V., et al. 2009. J. Immunol. 183:5705. PubMed


Description:

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Cy7 is useful for detecting biotinylated antibodies. The excitation of APC/Cy7 by 600-635 nm laser light induces a fluorescence emission maximum of 780 nm.

Other Names: Streptavidin-Allophycocyanin/Cy7, SAv-APC/Cy7
Cell Staining Buffer (FBS)FC,ICC,ICFC