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APC Streptavidin
APC Streptavidin
405207 100 µg $100.00     
Product Details
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Streptavidin is conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated Streptavidin.
Concentration: 0.2 mg/ml
Storage & Handling: The Streptavidin-APC solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested
Recommended Usage:

Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
Notes:

Streptavidin-Allophycocyanin (APC) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application
References:

1. Kamimura D, et al. 2006. J. Immunol. 177:306.
2. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
3. Chou WC,et al.2006.Blood108:3005. PubMed
4. Malagolini N, et al. 2009. Glycobiology. 19:172. PubMed
5. Kroeger KM, et al. 2009. J. Leukocyte Biol. PubMed
6. Imada M, et al. 2009. Int. Immunol. 21:1151. PubMed
7. Yi Y, et al. 2010 J. Biol Chem. 285:21233. PubMed
8. King IL, et al. 2010. J. Immunol. 185:6138. PubMed
9. Yokoyama T, et al. 2011. Int Immunol. PubMed.
10. Rusert P, et al. 2011. J. Exp Med. 208:1419. PubMed.




Description:

Streptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm.
Other Names: Streptavidin-Allophycocyanin, SAv-APC
GeneID: NA
Latest Publications: View the latest Streptavidin articles on HighwirePress.com
UniProt: View information about Streptavidin on UniProt.org
Keywords: APC Streptavidin, APC, Streptavidin-Allophycocyanin, SAv-APC, Mouse, Human, Rat, Immunology, Antibodies
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