Streptavidin is conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated Streptavidin.
Concentration:
0.2 mg/ml
Storage & Handling:
The Streptavidin-APC solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC, ICFC
Recommended Usage:
Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin-Allophycocyanin (APC) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.
Application References:
1. Kamimura, D., et al., 2006. J. Immunol. 177: 306. 2. Andoniou, C. E., et al., 2005. Nature Immunology 6: 1011. 3. Chou, WC.,et al.2006.Blood.108:3005.PubMed 4. Malagolini, N., et al. 2009. Glycobiology. 19:172. PubMed 5. Kroeger, KM., et al. 2009. J Leukoc Biol. PubMed 6. Imada, M., et al. 2009. Int. Immunol. 21:1151. PubMed
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm.
