Each lot of this Streptavidin-AKP is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:200 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin-AKP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Sav-AKP is recommended for ELISA and Western blotting, when used with the relevant substrate system.
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-AKP is useful for detecting biotinylated antibodies, e.g., in ELISA, ELISPOT, IHC or Western blotting. For ELISA applications, AKP will act on soluble substrates, such as pNPP, to yield a colorimetric reaction. For ELISPOT or Western blotting applications, AKP will act on precipitating substrates, such as NBT/BCIP, to yield a colorimetric reaction.
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