Each lot of this Streptavidin-AKP is quality control tested by ELISA assay. For ELISA or Western blot analysis, the reagent should be titrated between 1:200 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Streptavidin-AKP is useful as a second step reagent for indirect enzymatic labelling, in conjunction with biotinylated primary antibodies. Sav-AKP is recommended for ELISA and Western blotting, when used with the relevant substrate system.
Description:
Streptavidin binds to biotin with high affinity. Streptavidin-AKP is useful for detecting biotinylated antibodies, e.g., in ELISA, ELISPOT, IHC or Western blotting. For ELISA applications, AKP will act on soluble substrates, such as pNPP, to yield a colorimetric reaction. For ELISPOT or Western blotting applications, AKP will act on precipitating substrates, such as NBT/BCIP, to yield a colorimetric reaction.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering/). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
